Mouse Galectin-1 ELISA Kit | LGALS1 elisa kit

Galectin-1 Mouse ELISA Kit

Cat. Num. AAA355241

• Gene Names: LGALS1; GBP; GAL1
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Galectin-1; Galectin-1 Mouse ELISA Kit; Mouse Galectin-1; LGALS1 elisa kit

• Reactivity: Mouse

• Applicable Applications for LGALS1 elisa kit: Quantitative sELISA (EIA)
• Application Notes: For quantitative detection of Galectin-1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.
• Assay Type: Sandwich
• Detection Range: 156 pg/ml-10,000 pg/ml
• Sensitivity: < 5pg/ml
• Preparation and Storage: Store at 2-8 degree C for 4 months, or at -20 degree C for 8 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- Galectin-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GALECTIN-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Galectin-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Galectin-1 can be calculated.

Background: Galectin-1 is a member of the galectins, which are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. In Mouses it is encoded by the LGALS1 gene, which maps to 22q12-q13. It is expressed by the endometrial stromal cells throughout the menstrual cycle, however significantly increases during implantation. The Galectin-a plays a role in the immunosuppression required for a successful pregnancy, and it may also act as an autocrine negative growth factor that regulates cell proliferation.

NCBI and Uniprot Product Information

• NCBI GI #: 4504981
• NCBI GeneID: 3956
• NCBI Accession #: NP_002296.1
• NCBI GenBank Nucleotide #: NM_002305.3
• UniProt Accession #: P09382; Q9UDK5; B2R5E8
• Molecular Weight: 14,716 Da
• NCBI Official Full Name: galectin-1
• NCBI Official Synonym Full Names: lectin, galactoside-binding, soluble, 1
• NCBI Official Symbol: LGALS1
• NCBI Official Synonym Symbols: GBP; GAL1
• NCBI Protein Information: galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa
• UniProt Protein Name: Galectin-1
• Protein Family: Galectin
• UniProt Gene Name: LGALS1
• UniProt Synonym Gene Names: Gal-1; HLBP14
• UniProt Entry Name: LEG1_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Ref.24 Ref.29

Subunit structure: Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N-acetyllactosamine). Ref.18 Ref.23 Ref.28 Ref.29

Subcellular location: Secreted › extracellular space › extracellular matrix Ref.24.

Tissue specificity: Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells. Ref.4

Sequence similarities: Contains 1 galectin domain.