Rat Nucleobindin 2 (NUCB2) ELISA Kit | NUCB2 elisa kit

Rat Nucleobindin 2 (NUCB2) ELISA Kit

Cat. Num. AAA2704955

• Gene Names: NUCB2; NEFA; HEL-S-109
• Reactivity: Rat
• Synonyms: Nucleobindin 2 (NUCB2); Rat Nucleobindin 2 (NUCB2) ELISA Kit; NUCB2; NEFA; Nucleobindin 2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin; NUCB2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nucleobindin 2 (NUCB2).; No significant cross-reactivity or interference between Nucleobindin 2 (NUCB2) and analogues was observed.
• Sequence Length: 420

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.102ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nucleobindin 2 (NUCB2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nucleobindin 2 (NUCB2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NUCB2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nucleobindin 2 (NUCB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nucleobindin 2 (NUCB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nucleobindin 2 (NUCB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nucleobindin 2 (NUCB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4826870
• NCBI GeneID: 4925
• NCBI Accession #: NP_005004.1
• NCBI GenBank Nucleotide #: NM_005013.3
• UniProt Accession #: P80303; Q8NFT5; A8K642; D3DQX5
• Molecular Weight: 50,201 Da
• NCBI Official Full Name: nucleobindin-2 isoform 1 preproprotein
• NCBI Official Synonym Full Names: nucleobindin 2
• NCBI Official Symbol: NUCB2
• NCBI Official Synonym Symbols: NEFA; HEL-S-109
• NCBI Protein Information: nucleobindin-2
• UniProt Protein Name: Nucleobindin-2
• Protein Family: Nucleobindin
• UniProt Gene Name: NUCB2
• UniProt Synonym Gene Names: NEFA

NCBI Description

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

Uniprot Description

Calcium-binding protein. May have a role in calcium homeostasis.Nesfatin-1: Anorexigenic peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance ().