Mouse Nucleobindin-2 ELISA Kit | Nucb2 elisa kit

Mouse Nucleobindin-2 ELISA Kit

Cat. Num. AAA761382

• Gene Names: NUCB2; NEFA; HEL-S-109
• Reactivity: Mouse
• Synonyms: Nucleobindin-2; Mouse Nucleobindin-2 ELISA Kit; Nucb2/NEFA/Prepronesfatin/DNA-binding protein NEFA/Gastric cancer antigen Zg4/nucleobindin 2; Nucb2 elisa kit

• Reactivity: Mouse
• Sequence Length: 420

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Nucb2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Nucb2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Nucb2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Nucb2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Nucb2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4826870
• NCBI GeneID: 4925
• NCBI Accession #: NP_005004.1
• NCBI GenBank Nucleotide #: NM_005013.2
• Molecular Weight: 50,201 Da
• NCBI Official Full Name: nucleobindin-2
• NCBI Official Synonym Full Names: nucleobindin 2
• NCBI Official Symbol: NUCB2
• NCBI Official Synonym Symbols: NEFA; HEL-S-109
• NCBI Protein Information: nucleobindin-2
• UniProt Protein Name: Nucleobindin-2
• Protein Family: Nucleobindin
• UniProt Gene Name: NUCB2
• UniProt Synonym Gene Names: NEFA
• UniProt Entry Name: NUCB2_HUMAN

NCBI Description

This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]

Uniprot Description

NUCB2: Calcium-binding protein. May have a role in calcium homeostasis. Belongs to the nucleobindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.1

Cellular Component: cytosol; endoplasmic reticulum; ER-Golgi intermediate compartment; extracellular space; Golgi apparatus; nuclear envelope; plasma membrane

Molecular Function: calcium ion binding; DNA binding; protein binding