Rat NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1) ELISA Kit | NFATC1 elisa kit

Rat NFATC1 (Nuclear factor of activated T-cells, cytoplasmic 1) ELISA Kit

Cat. Num. AAA7606578

• Gene Names: Nfatc1; NFAT2; NFATc; NF-ATc; Nfatcb; AI449492; AV076380; 2210017P03Rik
• Reactivity: Rat
• Synonyms: NFATC1 (Nuclear factor of activated T-cells; cytoplasmic 1); Rat NFATC1 (Nuclear factor of activated T-cells; cytoplasmic 1) ELISA Kit; Nuclear factor of activated T-cells; cytoplasmic 1; NFATC1 elisa kit

• Reactivity: Rat
• Sequence Length: 827

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NFATC1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- NFATC1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-NFATC1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the NFATC1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of NFATC1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38348193
• NCBI GeneID: 18018
• NCBI Accession #: NP_940821.1
• NCBI GenBank Nucleotide #: NM_198429.2
• UniProt Accession #: O88942; O70345
• Molecular Weight: 74,328 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform 2
• NCBI Official Synonym Full Names: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1
• NCBI Official Symbol: Nfatc1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATc; Nfatcb; AI449492; AV076380; 2210017P03Rik
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: Nfatc1
• UniProt Synonym Gene Names: Nfat2; Nfatc; NF-ATc1; NFATc1; NF-ATc; NFATc

Uniprot Description

NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18 E3|18 53.66 cM

Cellular Component: cytoplasm; nuclear body; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin binding; DNA binding; mitogen-activated protein kinase p38 binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor binding

Biological Process: B-1a B cell differentiation; calcineurin-NFAT signaling pathway; calcium ion transport; epithelial to mesenchymal transition; G1/S transition of mitotic cell cycle; heart development; lymphangiogenesis; negative regulation of osteoblast differentiation; negative regulation of transcription from RNA polymerase II promoter; osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of hair cycle; regulation of transcription from RNA polymerase II promoter; transition between fast and slow fiber