Typical Testing Data/Standard Curve (for reference only)
Mouse Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit | NPY5R elisa kit
Mouse Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit
Gene Names
NPY5R; NPYR5; NPY5-R; NPYY5-R
Reactivity
Mouse
Synonyms
Neuropeptide Y Receptor Y5 (NPY5R); Mouse Neuropeptide Y Receptor Y5 (NPY5R) ELISA Kit; NPYR5; NPY-Y5 receptor; NPY5R elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Neuropeptide Y Receptor Y5 (NPY5R).
No significant cross-reactivity or interference between Neuropeptide Y Receptor Y5 (NPY5R) and analogues was observed.
No significant cross-reactivity or interference between Neuropeptide Y Receptor Y5 (NPY5R) and analogues was observed.
Sequence Length
445
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates and Other Biological Fluids
Detection Range
0.156-10ng/mL
Sensitivity
< 0.057ng/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Neuropeptide Y Receptor Y5 (NPY5R) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Neuropeptide Y Receptor Y5 (NPY5R) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NPY5R elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuropeptide Y Receptor Y5 (NPY5R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Neuropeptide Y Receptor Y5 (NPY5R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuropeptide Y Receptor Y5 (NPY5R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuropeptide Y Receptor Y5 (NPY5R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for NPY5R elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,727 Da
NCBI Official Full Name
neuropeptide Y receptor Y5
NCBI Official Synonym Full Names
neuropeptide Y receptor Y5
NCBI Official Symbol
NPY5R
NCBI Official Synonym Symbols
NPYR5; NPY5-R; NPYY5-R
NCBI Protein Information
neuropeptide Y receptor type 5
UniProt Protein Name
Neuropeptide Y receptor type 5
Protein Family
UniProt Gene Name
NPY5R
UniProt Synonym Gene Names
NPYR5; NPY5-R; NPYY5-R; Y5 receptor
UniProt Entry Name
NPY5R_HUMAN
Uniprot Description
NPY5R: Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 4q31-q32
Cellular Component: neuron projection; integral to plasma membrane; cytoplasm; plasma membrane
Molecular Function: peptide YY receptor activity; pancreatic polypeptide receptor activity; neuropeptide Y receptor activity
Biological Process: generation of ovulation cycle rhythm; synaptic transmission; G-protein coupled receptor protein signaling pathway; negative regulation of glutamate secretion; eating behavior; neuropeptide signaling pathway; positive regulation of acute inflammatory response; positive regulation of smooth muscle cell proliferation; negative regulation of synaptic transmission, GABAergic; negative regulation of apoptosis
Research Articles on NPY5R
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Product Notes
The Mouse NPY5R npy5r (Catalog #AAA2019860) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2019860 ELISA Kit recognizes Mouse NPY5R. It is sometimes possible for the material contained within the vial of "Neuropeptide Y Receptor Y5 (NPY5R), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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