Human Neuropeptide Y Receptor Y5 ELISA Kit | NPYR5 elisa kit

Human Neuropeptide Y Receptor Y5 ELISA Kit

Cat. Num. AAA765469

• Gene Names: NPY5R; NPYR5; NPY5-R; NPYY5-R
• Reactivity: Human
• Synonyms: Neuropeptide Y Receptor Y5; Human Neuropeptide Y Receptor Y5 ELISA Kit; NPYR5; NPYR5 elisa kit

• Reactivity: Human
• Sequence Length: 445

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NPYR5 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NPYR5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NPYR5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NPYR5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NPYR5 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 32481997
• NCBI GeneID: 4889
• Molecular Weight: 50,727 Da
• NCBI Official Full Name: neuropeptide Y receptor Y5
• NCBI Official Synonym Full Names: neuropeptide Y receptor Y5
• NCBI Official Symbol: NPY5R
• NCBI Official Synonym Symbols: NPYR5; NPY5-R; NPYY5-R
• NCBI Protein Information: neuropeptide Y receptor type 5
• UniProt Protein Name: Neuropeptide Y receptor type 5
• UniProt Gene Name: NPY5R
• UniProt Synonym Gene Names: NPYR5; NPY5-R; NPYY5-R; Y5 receptor
• UniProt Entry Name: NPY5R_HUMAN

NCBI Description

The protein encoded by this gene is a receptor for neuropeptide Y and peptide YY. The encoded protein appears to be involved in regulating food intake, with defects in this gene being associated with eating disorders. Also, the encoded protein is involved in a pathway that protects neuroblastoma cells from chemotherapy-induced cell death, providing a possible therapeutic target against neuroblastoma. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Nov 2015]

Uniprot Description

NPY5R: Receptor for neuropeptide Y and peptide YY. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. Seems to be associated with food intake. Could be involved in feeding disorders. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 4q31-q32

Cellular Component: cytoplasm; integral to plasma membrane; neuron projection; plasma membrane

Molecular Function: neuropeptide Y receptor activity; pancreatic polypeptide receptor activity; peptide YY receptor activity

Biological Process: cell surface receptor linked signal transduction; eating behavior; feeding behavior; G-protein coupled receptor protein signaling pathway; generation of ovulation cycle rhythm; negative regulation of apoptosis; negative regulation of glutamate secretion; negative regulation of synaptic transmission, GABAergic; neuropeptide signaling pathway; positive regulation of acute inflammatory response; positive regulation of smooth muscle cell proliferation; synaptic transmission