Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuronal migration protein doublecortin (DCX) Standard Curve.)
Neuronal Migration Protein Doublecortin (DCX) ELISA Kit | DCX elisa kit
Rat Neuronal Migration Protein Doublecortin (DCX) ELISA Kit
Reactivity
Rat
Synonyms
Neuronal Migration Protein Doublecortin (DCX); Rat Neuronal Migration Protein Doublecortin (DCX) ELISA Kit; RP5-914P14.1; DBCN; DC; LISX; SCLH; XLIS; doublecortex; doublin; lissencephalin-X; DCX elisa kit
Reactivity
Rat
Specificity
Rat Neuronal migration protein doublecortin (DCX) ELISA Kit has high sensitivity and excellent specificity for detection of Rat DCX. No significant cross-reactivity or interference between Rat DCX and analogues was observed.
Sequence Length
365
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuronal migration protein doublecortin (DCX) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Neuronal migration protein doublecortin (DCX) Standard Curve.)
Related Product Information for DCX elisa kit
Description: This Rat Neuronal migration protein doublecortin (DCX) ELISA Kit employs a two-site sandwich ELISA to quantitate DCX in samples. An antibody specific for DCX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DCX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DCX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DCX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: DCX encodes a member of the doublecortin family. The protein encoded by DCX is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in DCX cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for DCX.
Background: DCX encodes a member of the doublecortin family. The protein encoded by DCX is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in DCX cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for DCX.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
NCBI Official Full Name
Neuronal migration protein doublecortin
UniProt Protein Name
Neuronal migration protein doublecortin
Protein Family
UniProt Gene Name
Dcx
Uniprot Description
Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration.
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Product Notes
The Rat DCX dcx (Catalog #AAA9712460) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712460 ELISA Kit recognizes Rat DCX. It is sometimes possible for the material contained within the vial of "Neuronal Migration Protein Doublecortin (DCX), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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