Rat Doublecortin (DCX) RTU ELISA Kit | DCX rtu elisa kit

Rat Doublecortin (DCX) ELISA Kit

Cat. Num. AAA458801

• Reactivity: Rat
• Synonyms: Doublecortin (DCX); Rat Doublecortin (DCX) ELISA Kit; DBCN; DC; LISX; SCLH; XLIS; Doublin; Doublecortex; Lissencephalin-X; Lissencephaly; X-Linked; Neuronal migration protein doublecortin; DCX rtu elisa kit

• Reactivity: Rat
• Sequence Length: 365

• Assay Type: Sandwich
• Samples: Tissue Homogenates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.054ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DCX rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DCX. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DCX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DCX, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of DCX in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 59797481
• NCBI GeneID: 84394
• UniProt Accession #: Q9ESI7
• Molecular Weight: 40,560 Da
• NCBI Official Full Name: Neuronal migration protein doublecortin
• NCBI Official Synonym Full Names: doublecortin
• NCBI Official Symbol: Dcx
• NCBI Protein Information: neuronal migration protein doublecortin
• UniProt Protein Name: Neuronal migration protein doublecortin
• Protein Family: Protein doublecortin
• UniProt Gene Name: Dcx

NCBI Description

This gene encodes a member of the doublecortin family. The protein encoded by this gene contains two doublecortin domains, which bind microtubules and regulate microtubule polymerization. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to direct neuronal migration by regulating the organization and stability of microtubules. Studies in knockout mice lacking this gene suggest that this gene has a cortical role in nuclear translocation and positioning of the mitotic spindle in radial glial mitotic division. This gene is essential for neuronal migration, differentiation, and plasticity, is required for hippocampal development and also plays a role in dendrites development.[provided by RefSeq, Sep 2010]

Uniprot Description

Microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May participate along with PAFAH1B1/LIS-1 in a distinct overlapping signaling pathway that promotes neuronal migration.