Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit | NPR3 elisa kit

Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit

Cat. Num. AAA2704889

• Reactivity: Rat
• Synonyms: Natriuretic Peptide Receptor 3 (NPR3); Rat Natriuretic Peptide Receptor 3 (NPR3) ELISA Kit; GUCY2B; NPRC; ANPRC; Guanylate Cyclase C; Atrionatriuretic Peptide Receptor C; Atrial natriuretic peptide clearance receptor; NPR3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Natriuretic Peptide Receptor 3 (NPR3).; No significant cross-reactivity or interference between Natriuretic Peptide Receptor 3 (NPR3) and analogues was observed.
• Sequence Length: 536

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Natriuretic Peptide Receptor 3 (NPR3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Natriuretic Peptide Receptor 3 (NPR3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NPR3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Natriuretic Peptide Receptor 3 (NPR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Natriuretic Peptide Receptor 3 (NPR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Natriuretic Peptide Receptor 3 (NPR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Natriuretic Peptide Receptor 3 (NPR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6002707
• Molecular Weight: 59,808 Da
• NCBI Official Full Name: natriuretic peptide receptor 3
• UniProt Protein Name: Atrial natriuretic peptide receptor 3
• Protein Family: Nitrogen permease regulator
• UniProt Gene Name: Npr3
• UniProt Synonym Gene Names: ANP-C; ANPR-C; NPR-C

Uniprot Description

Receptor for the natriuretic peptide hormones, binding with similar affinities atrial natriuretic peptide NPPA/ANP, brain natriuretic peptide NPPB/BNP, and C-type natriuretic peptide NPPC/CNP (PubMed:10377427, PubMed:17951249). May function as a clearance receptor for NPPA, NPPB and NPPC, regulating their local concentrations and effects (PubMed:10377427, PubMed:17951249). May regulate diuresis, blood pressure and skeletal development (PubMed:10377427). Does not have guanylate cyclase activity (PubMed:10377427).