Typical Testing Data/Standard Curve (for reference only)
Mouse N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit | NT-ProBNP elisa kit
Mini Samples N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to NT-ProBNP. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NT-ProBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of NT-ProBNP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a secreted protein that belongs to the family of natriuretic peptides. Its precursor protein is processed to generate the active mature peptide. The mature peptide is a cardiac hormone that plays a role in ventricular remodeling as well as blood pressure regulation. Mice lacking this gene exhibit cardiac fibrosis. In humans this gene is associated with congestive heart failure, low bone-mineral density and postmenopausal osteoporosis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
Uniprot Description
NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.
Protein type: Hormone; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4 E2|4 78.57 cM
Cellular Component: cytoplasm; extracellular region; extracellular space; nucleus; perinuclear region of cytoplasm; protein complex
Molecular Function: hormone activity; peptide hormone receptor binding
Biological Process: cardiac muscle hypertrophy in response to stress; cGMP biosynthetic process; negative regulation of cell growth; negative regulation of smooth muscle cell proliferation; negative regulation of systemic arterial blood pressure; regulation of blood vessel size
Research Articles on NT-ProBNP
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Product Notes
The Mouse NT-ProBNP nppb (Catalog #AAA2709397) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709397 ELISA Kit recognizes Mouse NT-ProBNP. AAA Biotech's N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked immunosorbent assay for Antigen Detection. Researchers should empirically determine the suitability of the NT-ProBNP nppb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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