Rabbit UCHL1 Polyclonal Antibody | anti-UCHL1 antibody

UCHL1 (R202) Polyclonal Antibody

Cat. Num. AAA3000809

• Gene Names: UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UCHL1; Polyclonal Antibody; UCHL1 (R202) Polyclonal Antibody; Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; anti-UCHL1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PGP9.5 (R202) polyclonal antibody detects endogenous levels of PGP9.5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 223

• Applicable Applications for anti-UCHL1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IP: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 171-220 of Human UCHL1.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UCHL1 (R202) polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:The Brain tissue lysate of Rat (40ug) Lane5:The Brain tissue lysate of Mouse (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of UCHL1 (R202) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.)

Immunoprecipitation (IP)

(Immunoprecipitation of the Testis tissue lysate of Rat using UCHL1 (R202) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 and lane 5). Lane 1 is 30% input. The Western Blot was probed using UCHL1 (R202) pAb # MBS3000809. “up-arrow” (supernatant) ; “down-arrow” (deposition))

Western Blot (WB)

(Western Blot (WB) analysis of UCHL1 (R202) pAb at 1:500 dilutionLane1:The Brain tissue lysate of Rat (40ug) Lane2:The Brain tissue lysate of Mouse (40ug) Lane3:HEK293T whole cell lysate (40ug) Lane4:Hela whole cell lysate (40ug))

Related Product Information for anti-UCHL1 antibody

UCHL1 is a single chain transmembraneous glycoprotein which represents the low molecular weight isoform of the Leucocyte Common Antigen (LCA). It is expressed on most thymocytes, about 45% of peripheral blood T cells, virtually all T cells in skin reactive infiltrates, and the majority of T cell malignancies. It is also found on a subset of B cells and on exceptional B cell lymphomas.

NCBI and Uniprot Product Information

• NCBI GI #: 21361091
• NCBI GeneID: 7345
• NCBI Accession #: NP_004172.2
• NCBI GenBank Nucleotide #: NP_004172.2
• UniProt Accession #: P09936
• Molecular Weight: ~ 25kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin C-terminal hydrolase L1
• NCBI Official Symbol: UCHL1
• NCBI Official Synonym Symbols: NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: UCHL1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_HUMAN

NCBI Description

The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: Cell development/differentiation; EC 3.4.19.12; Ligase; Protease; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 4p14

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; axon; cytoplasm; plasma membrane; cytosol

Molecular Function: protein binding; omega peptidase activity; ubiquitin binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; protein deubiquitination; axon transport of mitochondrion; axon target recognition; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior

Disease: Neurodegeneration With Optic Atrophy, Childhood-onset; Parkinson Disease 5, Autosomal Dominant