Rat MMP9 ELISA Kit | MMP9 elisa kit

Rat MMP-9 PicoKine ELISA Kit

Cat. Num. AAA178530

• Reactivity: Rat
• Synonyms: MMP9; Rat MMP-9 PicoKine ELISA Kit; 67 kDa matrix metalloproteinase-9; 82 kDa matrix metalloproteinase-9; 92 kDa gelatinase; 92 kDa type IV collagenase; CLG 4B; CLG4B; Collagenase type IV 92 KD; EC 3.4.24.35; Gelatinase 92 KD; Gelatinase B; Gelatinase beta; GELB; Macrophage gelatinase; MANDP2; Matrix metalloproteinase 9; Matrix metalloproteinase9; Matrix metalloproteinase-9; MMP 9; mmp 9 kit; MMP-9; P50282; matrix metallopeptidase 9; MMP9 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat MMP-9
• Sequence Length: 708

• Samples: Cell culture supernates, serum and plasma(heparin).
• Assay Type: Sandwich
• Detection Range: 156pg/ml-10000pg/ml
• Sensitivity: <15pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice).

Typical Testing Data/Standard Curve (for reference only)

(Rat MMP-9 PicoKine ELISA Kit standard curve )

Related Product Information for MMP9 elisa kit

Principle of the Assay: Rat MMP-9 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-9 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: L35-P708) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-9 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin- Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat MMP-9 amount of sample captured in plate.

Background/Introduction: Matrix metallopeptidase 9 (MMP-9) is also known as 92 kDa type IV collagenase, 92 kDa gelatinase or gelatinase B (GELB). The 72- and 92-kDa type IV collagenases are members of a group of secreted zinc metalloproteases. The matrix metalloproteinases (MMPs) are able to degrade the extracellular matrix and allow angiogenesis and tumor invasion. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells. The polymorphism of MMP-9 acts as a genetic factor for the development of smoking-induced pulmonary emphysema.

NCBI and Uniprot Product Information

• NCBI GI #: 13591993
• NCBI GeneID: 81687
• NCBI Accession #: NP_112317.1
• NCBI GenBank Nucleotide #: NM_031055.1
• UniProt Accession #: P50282
• Molecular Weight: 78,611 Da
• NCBI Official Full Name: matrix metalloproteinase-9
• NCBI Official Synonym Full Names: matrix metallopeptidase 9
• NCBI Official Symbol: Mmp9
• NCBI Protein Information: matrix metalloproteinase-9
• UniProt Protein Name: Matrix metalloproteinase-9
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp9
• UniProt Synonym Gene Names: MMP-9; GELB
• UniProt Entry Name: MMP9_RAT

NCBI Description

metalloproteinase involved in extracellular matrix remodeling and bone resorption [RGD, Feb 2006]

Uniprot Description

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments ().