Western Blot (WB)
(Western blot (WB) analysis of ATG2B (D2078) polyclonal antibody at 1:500 dilution
Lane1:Myla2059 whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug))
Rabbit anti-Human, Rat ATG2B Polyclonal Antibody | anti-ATG2B antibody
ATG2B (D2078) polyclonal antibody
Gene Names
ATG2B; C14orf103
Reactivity
Human, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Synonyms
ATG2B; Polyclonal Antibody; ATG2B (D2078) polyclonal antibody; Autophagy-related protein 2 homolog B; anti-ATG2B antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
ATG2B (D2078) polyclonal antibody detects endogenous levels of ATG2B protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-ATG2B antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
WB: 1:500~1:1000
IP: 1:50~1:200
IHC: 1:50~1:200
IP: 1:50~1:200
IHC: 1:50~1:200
Immunogen
Synthetic peptide, corresponding to Human ATG2B.
Preparation and Storage
Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.
Western Blot (WB)
(Western blot (WB) analysis of ATG2B (D2078) polyclonal antibody at 1:500 dilution Lane1:Myla2059 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:C6 whole cell lysate(40ug))
Western Blot (WB)
(Western blot (WB) analysis of ATG2B (D2078) polyclonal antibody at 1:500 dilution
Lane1:Myla2059 whole cell lysate(40ug)
Lane2:PC3 whole cell lysate(40ug)
Lane3:HEK293T whole cell lysate(40ug)
Lane4:C6 whole cell lysate(40ug))
Immunoprecipitation (IP)
(Immunoprecipitation of 293T cell lysates using ATG2B (D2078) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using ATG2B (D2078) pAb #AP0776.)
Immunoprecipitation (IP)
(Immunoprecipitation of 293T cell lysates using ATG2B (D2078) pAb (Sepharose Bead Conjugate)#BD0048 (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (lane 4 and lane 5) .Lane 1 is 30% input. The western blot was probed using ATG2B (D2078) pAb #AP0776.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human Ovarian cancer using ATG2B antibody at dilution of 1:50.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded Human Ovarian cancer using ATG2B antibody at dilution of 1:50.)
Related Product Information for anti-ATG2B antibody
ATG2B, required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
229 kDa
NCBI Official Full Name
autophagy-related protein 2 homolog B
NCBI Official Synonym Full Names
autophagy related 2B
NCBI Official Symbol
ATG2B
NCBI Official Synonym Symbols
C14orf103
NCBI Protein Information
autophagy-related protein 2 homolog B; ATG2 autophagy related 2 homolog B
UniProt Protein Name
Autophagy-related protein 2 homolog B
Protein Family
UniProt Gene Name
ATG2B
UniProt Synonym Gene Names
C14orf103
UniProt Entry Name
ATG2B_HUMAN
Uniprot Description
ATG2B: Required for both autophagosome formation and regulation of lipid droplet morphology and dispersion. Belongs to the ATG2 family.
Protein type: Autophagy
Chromosomal Location of Human Ortholog: 14q32.2
Cellular Component: extrinsic to membrane; pre-autophagosomal structure membrane; lipid particle
Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
Research Articles on ATG2B
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Product Notes
The ATG2B atg2b (Catalog #AAA3004456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ATG2B (D2078) polyclonal antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). WB: 1:500~1:1000 IP: 1:50~1:200 IHC: 1:50~1:200. Researchers should empirically determine the suitability of the ATG2B atg2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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