Rat Mixed Lineage Kinase Domain Like Protein (MLKL) ELISA Kit | MLKL elisa kit

Rat Mixed Lineage Kinase Domain Like Protein (MLKL) ELISA Kit

Cat. Num. AAA2098151

• Gene Names: MLKL; hMLKL
• Reactivity: Rat
• Synonyms: Mixed Lineage Kinase Domain Like Protein (MLKL); Rat Mixed Lineage Kinase Domain Like Protein (MLKL) ELISA Kit; MLKL elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mixed Lineage Kinase Domain Like Protein (MLKL).; No significant cross-reactivity or interference between Mixed Lineage Kinase Domain Like Protein (MLKL) and analogues was observed.
• Sequence Length: 471

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mixed Lineage Kinase Domain Like Protein (MLKL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mixed Lineage Kinase Domain Like Protein (MLKL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MLKL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mixed Lineage Kinase Domain Like Protein (MLKL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mixed Lineage Kinase Domain Like Protein (MLKL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mixed Lineage Kinase Domain Like Protein (MLKL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mixed Lineage Kinase Domain Like Protein (MLKL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MLKL elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 22749323
• NCBI GeneID: 197259
• NCBI Accession #: NP_689862.1
• NCBI GenBank Nucleotide #: NM_152649.3
• UniProt Accession #: Q8NB16; Q8N6V0; A6NCE4
• Molecular Weight: 30,279 Da
• NCBI Official Full Name: mixed lineage kinase domain-like protein isoform 1
• NCBI Official Synonym Full Names: mixed lineage kinase domain like pseudokinase
• NCBI Official Symbol: MLKL
• NCBI Official Synonym Symbols: hMLKL
• NCBI Protein Information: mixed lineage kinase domain-like protein
• UniProt Protein Name: Mixed lineage kinase domain-like protein
• Protein Family: Mixed lineage kinase domain-like protein
• UniProt Gene Name: MLKL
• UniProt Synonym Gene Names: hMLKL

NCBI Description

This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

MLKL: Belongs to the protein kinase superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; TKL-Unique family

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: ATP binding; protein binding; protein complex binding; protein kinase activity; protein kinase binding; signal transducer activity

Biological Process: necroptotic process; protein amino acid phosphorylation