Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mediator of DNA damage checkpoint protein 1 (MDC1) Standard Curve.)
Rat Mediator Of DNA Damage Checkpoint Protein 1 (MDC1) ELISA Kit | MDC1 elisa kit
Rat Mediator Of DNA Damage Checkpoint Protein 1 (MDC1) ELISA Kit
Reactivity
Rat
Synonyms
Mediator Of DNA Damage Checkpoint Protein 1 (MDC1); Rat Mediator Of DNA Damage Checkpoint Protein 1 (MDC1) ELISA Kit; MDC1; DAAP-285E11.7; DKFZp781A0122; KIAA0170; MGC166888; NFBD1; homologue to Drosophila photoreceptor protein calphotin; mediator of DNA damage checkpoint 1; nuclear factor with BRCT domains 1; MDC1 elisa kit
Reactivity
Rat
Specificity
Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MDC1. No significant cross-reactivity or interference between Rat MDC1 and analogues was observed.
Sequence Length
1279
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mediator of DNA damage checkpoint protein 1 (MDC1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mediator of DNA damage checkpoint protein 1 (MDC1) Standard Curve.)
Related Product Information for MDC1 elisa kit
Description: This Rat Mediator of DNA damage checkpoint protein 1 (MDC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MDC1 in samples. An antibody specific for MDC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MDC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by MDC1 contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
Background: The protein encoded by MDC1 contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mediator of DNA damage checkpoint protein 1
NCBI Official Synonym Full Names
mediator of DNA damage checkpoint 1
NCBI Official Symbol
Mdc1
NCBI Protein Information
mediator of DNA damage checkpoint protein 1
UniProt Protein Name
Mediator of DNA damage checkpoint protein 1
Protein Family
UniProt Gene Name
Mdc1
UniProt Entry Name
MDC1_RAT
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Product Notes
The Rat MDC1 mdc1 (Catalog #AAA9712307) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712307 ELISA Kit recognizes Rat MDC1. It is sometimes possible for the material contained within the vial of "Mediator Of DNA Damage Checkpoint Protein 1 (MDC1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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