Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Matrix metalloproteinase 13 (MMP-13) Standard Curve.)
Rat Matrix Metalloproteinase 13 (MMP-13) ELISA Kit | MMP-13 elisa kit
Rat Matrix Metalloproteinase 13 (MMP-13) ELISA Kit
Reactivity
Rat
Synonyms
Matrix Metalloproteinase 13 (MMP-13); Rat Matrix Metalloproteinase 13 (MMP-13) ELISA Kit; MMP13; CLG3; MANDP1; collagenase 3; matrix metalloproteinase 13; matrix metalloproteinase 13 (collagenase 3); MMP-13 elisa kit
Reactivity
Rat
Specificity
Rat Matrix metalloproteinase 13 (MMP-13) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MMP13. No significant cross-reactivity or interference between Rat MMP13 and analogues was observed.
Sequence Length
471
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Matrix metalloproteinase 13 (MMP-13) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Matrix metalloproteinase 13 (MMP-13) Standard Curve.)
Related Product Information for MMP-13 elisa kit
Description: This Rat Matrix metalloproteinase 13 (MMP-13) ELISA Kit employs a two-site sandwich ELISA to quantitate MMP13 in samples. An antibody specific for MMP13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MMP13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP13 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MMP-13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP-13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions.MMP-13 is expressed by several metastatic tumors including breast carcinomas, chondrosarcomas, head and neck carcinomas, and in degenerative bone diseases including rheumatoid arthritis. Wild type and mutant p53, a tumor suppressor gene, differentially regulate MMP-13 gene expression. Members of the activator protein-1 and core-binding factor families increase MMP-13 promoter activity in normal, differentiating osteoblasts.
Background: MMP-13 has been proposed to participate in aggrecan degradation associated with osteoarthritis and cleavage of type II collagen in osteoarthritic cartilage explants and in tumor progression and metastasis. In addition, it can cleave type I, III, IV, IX, X and XIV collagens and fibronectin. MMP-13 is likely to play a crucial role in the modulation of extracellular matrix degradation and cell-matrix interactions.MMP-13 is expressed by several metastatic tumors including breast carcinomas, chondrosarcomas, head and neck carcinomas, and in degenerative bone diseases including rheumatoid arthritis. Wild type and mutant p53, a tumor suppressor gene, differentially regulate MMP-13 gene expression. Members of the activator protein-1 and core-binding factor families increase MMP-13 promoter activity in normal, differentiating osteoblasts.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
collagenase 3
NCBI Official Symbol
MMP13
NCBI Protein Information
collagenase 3
UniProt Protein Name
Collagenase 3
UniProt Gene Name
MMP13
UniProt Synonym Gene Names
MMP-13
Uniprot Description
Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CTGF. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CTGF. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion ().
Research Articles on MMP-13
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Product Notes
The Rat MMP-13 mmp13 (Catalog #AAA9712509) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712509 ELISA Kit recognizes Rat MMP-13. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 13 (MMP-13), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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