Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) Standard Curve.)
Rat Mannosyl-Oligosaccharide 1, 2-alpha-Mannosidase IA (MAN1A1) ELISA Kit | MAN1A1 elisa kit
Rat Mannosyl-Oligosaccharide 1, 2-alpha-Mannosidase IA (MAN1A1) ELISA Kit
Gene Names
MAN1A1; MAN9; HUMM3; HUMM9
Reactivity
Rat
Synonyms
Mannosyl-Oligosaccharide 1; 2-alpha-Mannosidase IA (MAN1A1); Rat Mannosyl-Oligosaccharide 1; 2-alpha-Mannosidase IA (MAN1A1) ELISA Kit; MAN1A1; HUMM3; HUMM9; MAN9; Man9-mannosidase; alpha-1; 2-mannosidase IA; mannosyl-oligosaccharide 1; 2-alpha-mannosidase IA N/A; MAN1A1 elisa kit
Reactivity
Rat
Specificity
Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAN1A1. No significant cross-reactivity or interference between Rat MAN1A1 and analogues was observed.
Sequence Length
653
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) Standard Curve.)
Related Product Information for MAN1A1 elisa kit
Description: This Rat Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA (MAN1A1) ELISA Kit employs a two-site sandwich ELISA to quantitate MAN1A1 in samples. An antibody specific for MAN1A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAN1A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAN1A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAN1A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the removal of 3 distinct mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. Using an oligonucleotide probe derived from a pig liver Man(9)-mannosidase-specific cDNA template, Bause et al. (1993) isolated Man(9)-mannosidase from a human kidney cDNA library. The full-length cDNA predicted a 625-amino acid protein with a calculated molecular mass of 71 kD. Man(9)-mannosidase is a type II transmembrane protein with a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a noncleavable signal sequence, a large luminal catalytic domain, and 3 potential N-glycosylation sites.
Background: MAN1A1 encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the removal of 3 distinct mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. Using an oligonucleotide probe derived from a pig liver Man(9)-mannosidase-specific cDNA template, Bause et al. (1993) isolated Man(9)-mannosidase from a human kidney cDNA library. The full-length cDNA predicted a 625-amino acid protein with a calculated molecular mass of 71 kD. Man(9)-mannosidase is a type II transmembrane protein with a short cytoplasmic polypeptide tail, a single transmembrane domain acting as a noncleavable signal sequence, a large luminal catalytic domain, and 3 potential N-glycosylation sites.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
NCBI Official Synonym Full Names
mannosidase alpha class 1A member 1
NCBI Official Symbol
MAN1A1
NCBI Official Synonym Symbols
MAN9; HUMM3; HUMM9
NCBI Protein Information
mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
UniProt Protein Name
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IA
Protein Family
UniProt Gene Name
MAN1A1
UniProt Synonym Gene Names
Man9-mannosidase
UniProt Entry Name
MA1A1_HUMAN
NCBI Description
This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq, Jul 2012]
Uniprot Description
MAN1A1: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). Belongs to the glycosyl hydrolase 47 family.
Protein type: Endoplasmic reticulum; Calcium-binding; EC 3.2.1.113; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Hydrolase
Chromosomal Location of Human Ortholog: 6q22
Cellular Component: Golgi membrane; Golgi apparatus; membrane; endoplasmic reticulum; cytoplasm; ER-Golgi intermediate compartment; integral to membrane
Molecular Function: mannosyl-oligosaccharide 1,2-alpha-mannosidase activity; calcium ion binding; mannosidase activity
Biological Process: cellular protein metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification
Research Articles on MAN1A1
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Product Notes
The Rat MAN1A1 man1a1 (Catalog #AAA9712666) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712666 ELISA Kit recognizes Rat MAN1A1. It is sometimes possible for the material contained within the vial of "Mannosyl-Oligosaccharide 1, 2-alpha-Mannosidase IA (MAN1A1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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