Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Low Density Lipoprotein cholesterol (HDL-C) Standard Curve.)
Rat Low Density Lipoprotein Cholesterol (HDL-C) ELISA Kit | HDL-C elisa kit
Rat Low Density Lipoprotein Cholesterol (HDL-C) ELISA Kit
Reactivity
Rat
Synonyms
Low Density Lipoprotein Cholesterol (HDL-C); Rat Low Density Lipoprotein Cholesterol (HDL-C) ELISA Kit; LDL-C; HDL-C elisa kit
Reactivity
Rat
Specificity
Rat Low Density Lipoprotein cholesterol (HDL-C) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LDL-C. No significant cross-reactivity or interference between Rat LDL-C and analogues was observed.
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Low Density Lipoprotein cholesterol (HDL-C) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Low Density Lipoprotein cholesterol (HDL-C) Standard Curve.)
Related Product Information for HDL-C elisa kit
Description: This Rat Low Density Lipoprotein cholesterol (HDL-C) ELISA Kit employs a two-site sandwich ELISA to quantitate LDL-C in samples. An antibody specific for LDL-C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LDL-C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LDL-C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LDL-C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Low-density lipoprotein (LDL) is a type of lipoprotein that transports cholesterol and triglycerides from the liver to peripheral tissues. LDL is one of the five major groups of lipoproteins; these groups include chylomicrons, very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein, and high-density lipoprotein (HDL). Like all lipoproteins, LDL enables fats and cholesterol to move within the water based solution of the blood stream. LDL also regulates cholesterol synthesis at these sites. It commonly appears in the medical setting as part of a cholesterol blood test, and since high levels of LDL cholesterol can signal medical problems like cardiovascular disease, it is sometimes called "bad cholesterol" (as opposed to high-density lipoprotein (HDL), which is frequently referred to as the "good cholesterol").
Background: Low-density lipoprotein (LDL) is a type of lipoprotein that transports cholesterol and triglycerides from the liver to peripheral tissues. LDL is one of the five major groups of lipoproteins; these groups include chylomicrons, very low-density lipoprotein (VLDL), intermediate-density lipoprotein (IDL), low-density lipoprotein, and high-density lipoprotein (HDL). Like all lipoproteins, LDL enables fats and cholesterol to move within the water based solution of the blood stream. LDL also regulates cholesterol synthesis at these sites. It commonly appears in the medical setting as part of a cholesterol blood test, and since high levels of LDL cholesterol can signal medical problems like cardiovascular disease, it is sometimes called "bad cholesterol" (as opposed to high-density lipoprotein (HDL), which is frequently referred to as the "good cholesterol").
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Product Notes
The Rat HDL-C (Catalog #AAA9712681) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712681 ELISA Kit recognizes Rat HDL-C. It is sometimes possible for the material contained within the vial of "Low Density Lipoprotein Cholesterol (HDL-C), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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