Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Low-density lipoprotein receptor-related protein 10 (LRP10) Standard Curve.)
Human Low-Density Lipoprotein Receptor-Related Protein 10 (LRP10) ELISA Kit | LRP10 elisa kit
Human Low-Density Lipoprotein Receptor-Related Protein 10 (LRP10) ELISA Kit
Gene Names
LRP10; LRP9; LRP-10; MST087; MSTP087
Reactivity
Human
Synonyms
Low-Density Lipoprotein Receptor-Related Protein 10 (LRP10); Human Low-Density Lipoprotein Receptor-Related Protein 10 (LRP10) ELISA Kit; LRP10; DKFZp564C1940; LRP9; MGC142274; MGC142276; MGC8675; MST087; MSTP087; LRP10 elisa kit
Reactivity
Human
Specificity
Human Low-density lipoprotein receptor-related protein 10 (LRP10) ELISA Kit has high sensitivity and excellent specificity for detection of Human LRP10. No significant cross-reactivity or interference between Human LRP10 and analogues was observed.
Sequence Length
713
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Low-density lipoprotein receptor-related protein 10 (LRP10) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Low-density lipoprotein receptor-related protein 10 (LRP10) Standard Curve.)
Related Product Information for LRP10 elisa kit
Description: This Human Low-density lipoprotein receptor-related protein 10 (LRP10) ELISA Kit employs a two-site sandwich ELISA to quantitate LRP10 in samples. An antibody specific for LRP10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRP10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRP10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRP10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Proteins with EGF-like domains often consist of more than 1,000 amino acids, have multiple copies of the EGF-like domain, and contain additional domains known to be involved in specific protein-protein interactions. The predicted partial LRP4 protein contains 2 EGF-like domains, a calcium binding-type EGF-like domain, 3 LDL receptor-type EGF-like domains, 4 YWTD spacer regions, a transmembrane domain, a cytoplasmic NPXY motif, which is required for clustering and internalization of LDL receptors, and a cytoplasmic tSXV motif, which anchors proteins with a PDZ domain. The sequence and domain organization of LRP4 shows significant similarities to those of members of the LDL receptor family. Northern blot analysis detected rat Megf7 expression in several regions of the brain.
Background: Proteins with EGF-like domains often consist of more than 1,000 amino acids, have multiple copies of the EGF-like domain, and contain additional domains known to be involved in specific protein-protein interactions. The predicted partial LRP4 protein contains 2 EGF-like domains, a calcium binding-type EGF-like domain, 3 LDL receptor-type EGF-like domains, 4 YWTD spacer regions, a transmembrane domain, a cytoplasmic NPXY motif, which is required for clustering and internalization of LDL receptors, and a cytoplasmic tSXV motif, which anchors proteins with a PDZ domain. The sequence and domain organization of LRP4 shows significant similarities to those of members of the LDL receptor family. Northern blot analysis detected rat Megf7 expression in several regions of the brain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
low-density lipoprotein receptor-related protein 10 isoform 1
NCBI Official Synonym Full Names
LDL receptor related protein 10
NCBI Official Symbol
LRP10
NCBI Official Synonym Symbols
LRP9; LRP-10; MST087; MSTP087
NCBI Protein Information
low-density lipoprotein receptor-related protein 10
UniProt Protein Name
Low-density lipoprotein receptor-related protein 10
Protein Family
UniProt Gene Name
LRP10
UniProt Synonym Gene Names
LRP-10
UniProt Entry Name
LRP10_HUMAN
NCBI Description
This gene encodes a low density lipoprotein receptor family protein. A similar protein in mouse is thought to play a role in the uptake of apolipoprotein E-containing lipoproteins. [provided by RefSeq, Jul 2016]
Uniprot Description
LRP10: Probable receptor, which is involved in the internalization of lipophilic molecules and/or signal transduction. May be involved in the uptake of lipoprotein APOE in liver. Belongs to the LDLR family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 14q11.2
Cellular Component: membrane; integral to membrane; coated pit
Biological Process: endocytosis
Research Articles on LRP10
Similar Products
Product Notes
The Human LRP10 lrp10 (Catalog #AAA9715448) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715448 ELISA Kit recognizes Human LRP10. It is sometimes possible for the material contained within the vial of "Low-Density Lipoprotein Receptor-Related Protein 10 (LRP10), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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