Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Latrophilin-3 (LPHN3) Standard Curve.)
Rat Latrophilin-3 (LPHN3) ELISA Kit | LPHN3 elisa kit
Rat Latrophilin-3 (LPHN3) ELISA Kit
Gene Names
Adgrl3; Lec3; Cirl3; Lphn3
Reactivity
Rat
Synonyms
Latrophilin-3 (LPHN3); Rat Latrophilin-3 (LPHN3) ELISA Kit; LPHN3; CIRL3; LEC3; calcium-independent alpha-latrotoxin receptor 3; latrophilin homolog 3 (cow); lectomedin 3; LPHN3 elisa kit
Reactivity
Rat
Specificity
Rat Latrophilin-3 (LPHN3) ELISA Kit has high sensitivity and excellent specificity for detection of Rat LPHN3. No significant cross-reactivity or interference between Rat LPHN3 and analogues was observed.
Sequence Length
1550
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Latrophilin-3 (LPHN3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Latrophilin-3 (LPHN3) Standard Curve.)
Related Product Information for LPHN3 elisa kit
Description: This Rat Latrophilin-3 (LPHN3) ELISA Kit employs a two-site sandwich ELISA to quantitate LPHN3 in samples. An antibody specific for LPHN3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LPHN3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LPHN3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LPHN3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Latrophilin-3 is a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin 3forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85)
Background: Latrophilin-3 is a member of the latrophilin subfamily of G-protein coupled receptors (GPCR). Latrophilins may function in both cell adhesion and signal transduction. In experiments with non-human species, endogenous proteolytic cleavage within a cysteine-rich GPS (G-protein-coupled-receptor proteolysis site) domain resulted in two subunits (a large extracellular N-terminal cell adhesion subunit and a subunit with substantial similarity to the secretin/calcitonin family of GPCRs) being non-covalently bound at the cell membrane. Latrophilin 3forms a heterodimer, consisting of a large extracellular region (p120) non-covalently linked to a seven-transmembrane moiety (p85)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
adhesion G protein-coupled receptor L3
NCBI Official Synonym Full Names
adhesion G protein-coupled receptor L3
NCBI Official Symbol
Adgrl3
NCBI Official Synonym Symbols
Lec3; Cirl3; Lphn3
NCBI Protein Information
adhesion G protein-coupled receptor L3
UniProt Protein Name
Latrophilin-3
UniProt Gene Name
Lphn3
UniProt Synonym Gene Names
Cirl3; Cl3; CIRL-3
UniProt Entry Name
LPHN3_RAT
NCBI Description
G-protein-coupled receptor [RGD, Feb 2006]
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Product Notes
The Rat LPHN3 lphn3 (Catalog #AAA9712356) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712356 ELISA Kit recognizes Rat LPHN3. It is sometimes possible for the material contained within the vial of "Latrophilin-3 (LPHN3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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