Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Lamin-B1 (LMNB1) Standard Curve.)
Rat Lamin-B1 (LMNB1) ELISA Kit | LMNB1 elisa kit
Rat Lamin-B1 (LMNB1) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Lamin-B1 (LMNB1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Lamin-B1 (LMNB1) Standard Curve.)
Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. LMNB1 encodes a deduced 586-amino acid protein with a calculated molecular mass of 66,334 Da. The LMNB1 protein shares approximately 72% sequence similarity with lamin A/C.
NCBI and Uniprot Product Information
NCBI Description
human homolog is a nuclear lamina protein; involved in nuclear stability, chromatin structure and gene expression [RGD, Feb 2006]
Uniprot Description
Lamin B1: nuclear lamins are intermediate filament proteins that constitute the lattice-like matrix at the inner face of the nuclear membrane that underlies the nuclear envelop. The lamins, highly conserved throughout evolution, are encoded by three genes in the human: LMNA, LMNB1, and LMNB2. The A-type lamins (lamin A/C) are developmentally regulated and are generally expressed in differentiated cells. The anchoring of chromatin to the nuclear lamina is involved in the control of gene expression and in DNA replication and repair. During mitosis, the nuclear lamina is reversibly disassembled as the lamin proteins are phosphorylated. B-type lamins are phosphorylated within minutes of engaging the IgM surface receptor of resting splenic B cells. Nuclear lamins are cleaved by caspases during apoptosis.
Protein type: Cytoskeletal
Cellular Component: cytoplasm; lamin filament; membrane; nuclear envelope; nuclear inner membrane; nuclear lamina; nuclear matrix; nuclear membrane; nucleoplasm; nucleus
Molecular Function: phospholipase binding; protein binding; structural molecule activity
Biological Process: apoptosis; neurogenesis; response to antibiotic; response to drug
Research Articles on LMNB1
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Product Notes
The Rat LMNB1 lmnb1 (Catalog #AAA9712359) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712359 ELISA Kit recognizes Rat LMNB1. It is sometimes possible for the material contained within the vial of "Lamin-B1 (LMNB1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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