Rat Kynurenine 3-monooxygenase (KMO) ELISA Kit | KMO elisa kit

Rat Kynurenine 3-monooxygenase (KMO) ELISA Kit

Cat. Num. AAA7211872

• Gene Names: KMO; dJ317G22.1
• Reactivity: Rat
• Synonyms: Kynurenine 3-monooxygenase (KMO); Rat Kynurenine 3-monooxygenase (KMO) ELISA Kit; KMO elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This KMO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat KMO. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KMO elisa kit

Principle of the assay: KMO ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-KMO antibody and an KMO-HRP conjugate. The assay sample and buffer are incubated together with KMO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KMO concentration since KMO from samples and KMO-HRP conjugate compete for the anti-KMO antibody binding site. Since the number of sites is limited, as more sites are occupied by KMO from the sample, fewer sites are left to bind KMO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KMO concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 187960045
• NCBI GeneID: 8564
• NCBI Accession #: NP_003670.2
• NCBI GenBank Nucleotide #: NM_003679.4
• Molecular Weight: 51,682 Da
• NCBI Official Full Name: kynurenine 3-monooxygenase
• NCBI Official Synonym Full Names: kynurenine 3-monooxygenase (kynurenine 3-hydroxylase)
• NCBI Official Symbol: KMO
• NCBI Official Synonym Symbols: dJ317G22.1
• NCBI Protein Information: kynurenine 3-monooxygenase; kynurenine 3-hydroxylase
• UniProt Protein Name: Kynurenine 3-monooxygenase
• UniProt Gene Name: KMO
• UniProt Entry Name: KMO_HUMAN

NCBI Description

This gene encodes a mitochondrion outer membrane protein that catalyzes the hydroxylation of L-tryptophan metabolite, L-kynurenine, to form L-3-hydroxykynurenine. Studies in yeast identified this gene as a therapeutic target for Huntington disease. [provided by RefSeq, Oct 2011]

Uniprot Description

KMO: Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract. Belongs to the aromatic-ring hydroxylase family. KMO subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Mitochondrial; EC 1.14.13.9; Amino Acid Metabolism - tryptophan; Oxidoreductase; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1q42-q44

Cellular Component: mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane; cytosol

Molecular Function: FAD binding; NAD(P)H oxidase activity; kynurenine 3-monooxygenase activity

Biological Process: anthranilate metabolic process; tryptophan catabolic process to kynurenine; NAD metabolic process; quinolinate biosynthetic process; tryptophan catabolic process; response to salt stress