Rat glucocorticoid receptor, GR ELISA Kit | GR elisa kit

Rat glucocorticoid receptor, GR ELISA Kit

Cat. Num. AAA161186

• Gene Names: NR3C1; GR; GCR; GRL; GCCR; GCRST
• Reactivity: Rat
• Synonyms: glucocorticoid receptor; GR; Rat glucocorticoid receptor; GR ELISA Kit; GR elisa kit

• Reactivity: Rat
• Sequence Length: 394

• Samples: Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.2ng/ml - 60ng/ml
• Sensitivity: 0.105ng/ml
• Intra-Assay: CV<8%
• Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GR elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Glucocorticoid Receptor (also known as GR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat GR antibody. GR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GR Antibody is added and binds to GR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated GR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat GR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 239758
• NCBI GeneID: 2908
• UniProt Accession #: P04150; P04151; Q53EP5; Q6N0A4; A0ZXF9; B0LPG8; D3DQF4; F5ATB7
• Molecular Weight: 81,509 Da
• NCBI Official Full Name: glucocorticoid receptor, partial
• NCBI Official Synonym Full Names: nuclear receptor subfamily 3 group C member 1
• NCBI Official Symbol: NR3C1
• NCBI Official Synonym Symbols: GR; GCR; GRL; GCCR; GCRST
• NCBI Protein Information: glucocorticoid receptor
• UniProt Protein Name: Glucocorticoid receptor
• UniProt Gene Name: NR3C1
• UniProt Synonym Gene Names: GRL; GR

NCBI Description

This gene encodes glucocorticoid receptor, which can function both as a transcription factor that binds to glucocorticoid response elements in the promoters of glucocorticoid responsive genes to activate their transcription, and as a regulator of other transcription factors. This receptor is typically found in the cytoplasm, but upon ligand binding, is transported into the nucleus. It is involved in inflammatory responses, cellular proliferation, and differentiation in target tissues. Mutations in this gene are associated with generalized glucocorticoid resistance. Alternative splicing of this gene results in transcript variants encoding either the same or different isoforms. Additional isoforms resulting from the use of alternate in-frame translation initiation sites have also been described, and shown to be functional, displaying diverse cytoplasm-to-nucleus trafficking patterns and distinct transcriptional activities (PMID:15866175). [provided by RefSeq, Feb 2011]

Uniprot Description

Receptor for glucocorticoids (GC) (PubMed:27120390). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Involved in chromatin remodeling (PubMed:9590696). Plays a role in rapid mRNA degradation by binding to the 5' UTR of target mRNAs and interacting with PNRC2 in a ligand-dependent manner which recruits the RNA helicase UPF1 and the mRNA-decapping enzyme DCP1A, leading to RNA decay (PubMed:25775514). Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth ().