Rat Kisspeptin 1 (KISS1) ELISA Kit | KISS1 elisa kit

Rat Kisspeptin 1 (KISS1) ELISA Kit

Cat. Num. AAA451194

• Gene Names: Kiss1; Eseptin
• Reactivity: Rat
• Synonyms: Kisspeptin 1 (KISS1); Rat Kisspeptin 1 (KISS1) ELISA Kit; Metastin; KiSS-1 Metastasis-Suppressor; KISS1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of KISS1. No significant cross-reactivity or interference between KISS1 and analogues was observed.
• Sequence Length: 130

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive inhibition
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 8.47pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KISS1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KISS1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KISS1 elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of KISS1 in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to KISS1 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled KISS1 and unlabeled KISS1 (Standards or samples) with the pre-coated antibody specific to KISS1. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of KISS1 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of KISS1 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 32140194
• NCBI GeneID: 289023
• NCBI Accession #: NP_859043.1
• NCBI GenBank Nucleotide #: NM_181692.1
• UniProt Accession #: Q7TSB7
• Molecular Weight: 14,188 Da
• NCBI Official Full Name: metastasis-suppressor KiSS-1
• NCBI Official Synonym Full Names: KiSS-1 metastasis-suppressor
• NCBI Official Symbol: Kiss1
• NCBI Official Synonym Symbols: Eseptin
• NCBI Protein Information: metastasis-suppressor KiSS-1
• UniProt Protein Name: Metastasis-suppressor KiSS-1
• Protein Family: Metastasis-suppressor
• UniProt Gene Name: Kiss1

NCBI Description

ligand for the G-protein-coupled receptor GPR54; may be involved in autonomic and sensory neural signaling [RGD, Feb 2006]

Uniprot Description

Metastasis suppressor protein. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Intracerebroventricular administration induces an increase in serum LH and FSH levels in prepubertal male and female as well as in adult animals.