Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Keratin, type I cytoskeletal 18 (KRT18) Standard Curve.)
Keratin, Type I Cytoskeletal 18 (KRT18) ELISA Kit | KRT18 elisa kit
Rat Keratin, Type I Cytoskeletal 18 (KRT18) ELISA Kit
Gene Names
Krt18; Krt1-18
Reactivity
Rat
Synonyms
Keratin; Type I Cytoskeletal 18 (KRT18); Rat Keratin; Type I Cytoskeletal 18 (KRT18) ELISA Kit; KRT18; CYK18; K18; cell proliferation-inducing protein 46; cytokeratin 18; KRT18 elisa kit
Reactivity
Rat
Specificity
Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KRT18. No significant cross-reactivity or interference between Rat KRT18 and analogues was observed.
Sequence Length
423
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Keratin, type I cytoskeletal 18 (KRT18) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Rat Keratin, type I cytoskeletal 18 (KRT18) Standard Curve.)
Related Product Information for KRT18 elisa kit
Description: This Rat Keratin, type I cytoskeletal 18 (KRT18) ELISA Kit employs a two-site sandwich ELISA to quantitate KRT18 in samples. An antibody specific for KRT18 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KRT18 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KRT18 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KRT18 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Human keratin 18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Endo B and its coexpressed type II keratin, Endo A (the mouse equivalent of human keratin 8), appear to be the first IF proteins expressed during murine development.
Background: Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Human keratin 18 and the homologous mouse Endo B are type I IF protein subunits whose expression is restricted in adults to a variety of simple epithelial tissues. Endo B and its coexpressed type II keratin, Endo A (the mouse equivalent of human keratin 8), appear to be the first IF proteins expressed during murine development.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
Krt18
NCBI Official Synonym Symbols
Krt1-18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
Protein Family
UniProt Gene Name
Krt18
UniProt Synonym Gene Names
CK-18; K18
NCBI Description
involved in testicular differentiation [RGD, Feb 2006]
Uniprot Description
When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection (). Involved in the uptake of thrombin-antithrombin complexes by hepatic cells.
Research Articles on KRT18
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Product Notes
The Rat KRT18 krt18 (Catalog #AAA9712383) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9712383 ELISA Kit recognizes Rat KRT18. It is sometimes possible for the material contained within the vial of "Keratin, Type I Cytoskeletal 18 (KRT18), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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