Immunohistochemistry (IHC)
(Sample Type: Mouse brainSample Type: Mouse Brain SlicesRed: primaryBlue: DAPIPrimaryDilution: 1:400Secondary Antibody: Anti-Rabbit IgG Alexa 594SecondaryDilution: 1:400Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt University of Seville)
Rabbit HSPA8 Polyclonal Antibody | anti-HSPA8 antibody
HSPA8 antibody - N-terminal region
Immunohistochemistry (IHC)
(Sample Type: Mouse brainSample Type: Mouse Brain SlicesRed: primaryBlue: DAPIPrimaryDilution: 1:400Secondary Antibody: Anti-Rabbit IgG Alexa 594SecondaryDilution: 1:400Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt University of Seville)
Immunohistochemistry (IHC)
(Sample Type: Mouse brainSample Type: Mouse Brain SlicesRed: primaryBlue: DAPIPrimaryDilution: 1:400Secondary Antibody: Anti-Rabbit IgG Alexa 594SecondaryDilution: 1:400Image Submitted By: Adahir Labrador-Garrido and Cintia Roodveldt University of Seville)
Western Blot (WB)
(Host: RabbitTarget Name: HIRIP3Sample Type: 293TAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: HIRIP3Sample Type: 293TAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: HSPA8Sample Tissue: Human Fetal LiverAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: HSPA8Sample Tissue: Human Fetal LiverAntibody Dilution: 1.0ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: HSPA8Sample Tissue: Human Jurkat Whole CellAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: HSPA8Sample Tissue: Human Jurkat Whole CellAntibody Dilution: 1ug/ml)
Western Blot (WB)
(Host: RabbitTarget Name: HSPA8Sample Type: Human 293T cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5.0 ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 12%)
Western Blot (WB)
(Host: RabbitTarget Name: HSPA8Sample Type: Human 293T cellLane A: Primary AntibodyLane B: Primary Antibody + Blocking PeptidePrimary Antibody Concentration: 1ug/mlPeptide Concentration: 5.0 ug/mlLysate Quantity: 25ug/lane/laneGel Concentration: 12%)
Western Blot (WB)
(Host: RabbitTarget Name: NOP56Sample Type: MCF7Antibody Dilution: 1.0ug/mlHSPA8 is strongly supported by BioGPS gene expression data to be expressed in MCF7)
Western Blot (WB)
(Host: RabbitTarget Name: NOP56Sample Type: MCF7Antibody Dilution: 1.0ug/mlHSPA8 is strongly supported by BioGPS gene expression data to be expressed in MCF7)
Western Blot (WB)
(Host: RabbitTarget Name: RHOT1Sample Type: JurkatAntibody Dilution: 1.0ug/mlHSPA8 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells)
Western Blot (WB)
(Host: RabbitTarget Name: RHOT1Sample Type: JurkatAntibody Dilution: 1.0ug/mlHSPA8 is strongly supported by BioGPS gene expression data to be expressed in Human Jurkat cells)
Western Blot (WB)
(HSPA8 antibody - N-terminal region validated by WB using Rat Brain lysate at 1:1,000.)
Western Blot (WB)
(HSPA8 antibody - N-terminal region validated by WB using Rat Brain lysate at 1:1,000.)
Western Blot (WB)
(WB Suggested Anti-HSPA8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
Western Blot (WB)
(WB Suggested Anti-HSPA8 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: 293T cell lysate)
Target Description: HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. The protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
Uniprot Description
HSC70: Acts as a repressor of transcriptional activation. Inhibits the transcriptional coactivator activity of CITED1 on Smad-mediated transcription. Chaperone. Isoform 2 may function as an endogenous inhibitory regulator of HSC70 by competing the co- chaperones. Interacts with HSPH1/HSP105. Interacts with IRAK1BP1. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Interacts with PACRG and TSC2. Interacts with BAG1. Interacts with SV40 VP1. Interacts with DNAJC7. Interacts with HERC5. Interacts with CITED1 (via N-terminus); the interaction suppresses the association of CITED1 to p300/CBP and Smad-mediated transcription transactivation. Constitutively synthesized. Ubiquitous. Belongs to the heat shock protein 70 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: RNA-binding; Chaperone; Heat shock protein; Nucleolus
Chromosomal Location of Human Ortholog: 11q24.1
Cellular Component: nucleoplasm; spliceosome; extracellular space; focal adhesion; membrane; melanosome; nucleolus; plasma membrane; intracellular; ribonucleoprotein complex; nucleus; cytosol; ubiquitin ligase complex
Molecular Function: protein binding; enzyme binding; G-protein-coupled receptor binding; heat shock protein binding; ubiquitin protein ligase binding; ATPase activity; unfolded protein binding; ATPase activity, coupled; ATP binding
Biological Process: axon guidance; viral reproduction; chaperone cofactor-dependent protein folding; protein folding; transcription, DNA-dependent; regulation of cell cycle; neurotransmitter secretion; RNA splicing; response to unfolded protein; nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; synaptic transmission; gene expression; protein refolding; post-Golgi vesicle-mediated transport; negative regulation of transcription, DNA-dependent
Research Articles on HSPA8
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Product Notes
The HSPA8 hspa8 (Catalog #AAA3209019) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSPA8 antibody - N-terminal region reacts with Cow, Goat, Human, Mouse, Pig, Rat, Sheep, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's HSPA8 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Researchers should empirically determine the suitability of the HSPA8 hspa8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Synthetic peptide located within the following region: MSKGPAVGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL. It is sometimes possible for the material contained within the vial of "HSPA8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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