Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type I cytoskeletal 14 (KRT14) Standard Curve.)
Human Keratin, Type I Cytoskeletal 14 (KRT14) ELISA Kit | KRT14 elisa kit
Human Keratin, Type I Cytoskeletal 14 (KRT14) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type I cytoskeletal 14 (KRT14) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Keratin, type I cytoskeletal 14 (KRT14) Standard Curve.)
Background: Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells.Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex and Dermatopathia pigmentosa reticularis, both of which are autosomal dominant mutations. KRT14 belongs to a large group of acidic type I keratins that interact with basic type II keratins to form the 8-nm cytoskeletal filaments of epithelial cells. Both type I and type II keratins have a central alpha-helical domain of over 300 amino acids that mediates keratin interaction. KRT14 is expressed in the basal layer of stratified squamous epithelia, including epidermis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
Uniprot Description
K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q21.2
Cellular Component: cytoplasm; keratin filament; intermediate filament; cytosol; nucleus
Molecular Function: protein binding; structural constituent of cytoskeleton
Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; hair cycle; aging
Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type
Research Articles on KRT14
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Product Notes
The Human KRT14 krt14 (Catalog #AAA9715548) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715548 ELISA Kit recognizes Human KRT14. It is sometimes possible for the material contained within the vial of "Keratin, Type I Cytoskeletal 14 (KRT14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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