Rat Kallikrein 5 (KLK5) ELISA Kit | KLK5 elisa kit

Rat Kallikrein 5 (KLK5) ELISA Kit

Cat. Num. AAA2019227

• Gene Names: KLK5; SCTE; KLKL2; KLK-L2
• Reactivity: Rat
• Synonyms: Kallikrein 5 (KLK5); Rat Kallikrein 5 (KLK5) ELISA Kit; KLK5 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of KLK5. No significant cross-reactivity or interference between KLK5 and analogues was observed.
• Sequence Length: 293

• Samples: Rat serum, plasma and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: The minimum detectable dose of KLK5 is typically less than 0.062ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level KLK5 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level KLK5 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of KLK5 in rat serum, plasma and other biological fluids.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLK5 elisa kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to KLK5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to KLK5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain KLK5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of KLK5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 117306176
• NCBI GeneID: 25818
• NCBI Accession #: NP_001070960.1
• NCBI GenBank Nucleotide #: NM_001077492.1
• UniProt Accession #: Q9Y337; Q53ZR3; Q9HBG8
• Molecular Weight: 32,019 Da
• NCBI Official Full Name: kallikrein-5 preproprotein
• NCBI Official Synonym Full Names: kallikrein-related peptidase 5
• NCBI Official Symbol: KLK5
• NCBI Official Synonym Symbols: SCTE; KLKL2; KLK-L2
• NCBI Protein Information: kallikrein-5; kallikrein 5; kallikrein-like protein 2; stratum corneum tryptic enzyme
• UniProt Protein Name: Kallikrein-5
• Protein Family: Kallikrein
• UniProt Gene Name: KLK5
• UniProt Synonym Gene Names: SCTE; KLK-L2
• UniProt Entry Name: KLK5_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK5: May be involved in desquamation. Belongs to the peptidase S1 family. Kallikrein subfamily.

Protein type: Protease; Secreted; EC 3.4.21.-; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: extracellular space

Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity

Biological Process: epidermis development; positive regulation of G-protein coupled receptor protein signaling pathway; proteolysis