Typical Testing Data/Standard Curve (for reference only)
Porcine Kallikrein 5 ELISA Kit | KLK5 elisa kit
Porcine Kallikrein 5 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: KLK5 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-KLK5 antibody and an KLK5-HRP conjugate. The assay sample and buffer are incubated together with KLK5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the KLK5 concentration since KLK5 from samples and KLK5-HRP conjugate compete for the anti-KLK5 antibody binding site. Since the number of sites is limited, as more sites are occupied by KLK5 from the sample, fewer sites are left to bind KLK5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLK5 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its expression is up-regulated by estrogens and progestins. The encoded protein is secreted and may be involved in desquamation in the epidermis. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
KLK5: May be involved in desquamation. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: Secreted, signal peptide; Secreted; EC 3.4.21.-; Protease
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: extracellular space
Molecular Function: peptidase activity; protein binding; serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: epidermis development; positive regulation of G-protein coupled receptor protein signaling pathway; proteolysis
Research Articles on KLK5
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Product Notes
The Porcine KLK5 klk5 (Catalog #AAA746922) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA746922 ELISA Kit recognizes Porcine KLK5. It is sometimes possible for the material contained within the vial of "Kallikrein 5, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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