Western Blot (WB)
(Western Blot analysis was performed on human samples (30 ug) of Alzheimer's Disease patient CSF sample (Lane 1) and corresponding normal CSF sample (Lane 2). The blots were probed with MBS600139 (2ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500). An ~110kD band corresponding to beta-Amyloid was observed in Alzheimer's Disease patient CSF sample and a 35kD band was observed across both)
Mouse anti-Human b-Amyloid Peptide Monoclonal Antibody
b-Amyloid Peptide
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
PurifiedPuri ed
Synonyms
b-Amyloid Peptide; Monoclonal Antibody; Anti -b-Amyloid Peptide; anti-b-Amyloid Peptide antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3G5
Specificity
Recognizes the 4-5kD b-amyloid peptide (b-AP). High concentrations of antibody will also react with A-a, B-a and g subunits of brinogen. Species Crossreactivity: human.
Purity/Purification
Purified
Puri ed
Puri ed
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
Applicable Applications for anti-b-Amyloid Peptide antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in ELISA, Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (alcohol-fixed, parrafin embedded, frozen): ~10ug/ml It is nessessary to pretreat the tissue with trypsin digestion.
Western Blot: 0.1-0.2ug/ml
ELISA: 0.5-1.0ug/ml
Dilution: Immunohistochemistry (alcohol-fixed, parrafin embedded, frozen): ~10ug/ml It is nessessary to pretreat the tissue with trypsin digestion.
Western Blot: 0.1-0.2ug/ml
ELISA: 0.5-1.0ug/ml
Immunogen
28aa synthetic peptide derived from the b-amyloid peptide (aa1-28).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis was performed on human samples (30 ug) of Alzheimer's Disease patient CSF sample (Lane 1) and corresponding normal CSF sample (Lane 2). The blots were probed with MBS600139 (2ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500). An ~110kD band corresponding to beta-Amyloid was observed in Alzheimer's Disease patient CSF sample and a 35kD band was observed across both)
Western Blot (WB)
(Western Blot analysis was performed on human samples (30 ug) of Alzheimer's Disease patient CSF sample (Lane 1) and corresponding normal CSF sample (Lane 2). The blots were probed with MBS600139 (2ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500). An ~110kD band corresponding to beta-Amyloid was observed in Alzheimer's Disease patient CSF sample and a 35kD band was observed across both)
Related Product Information for anti-b-Amyloid Peptide antibody
The progressive deposition of insoluble amyloid protein plaques in speci c areas of the brain and its blood vessels is associated with a number of neuropathologies and is an early invariant feature of Alzheimer's disease. The major component of these protein plaques is a peptide of 39-42 amino acids derived from a group of 695-770 amino acid precursor proteins collectively referred to as the b-amyloid precursor protein (Beta-APP). b-APP is highly conserved in vertebrates; however, its normal function has not been well de ned. In each of the b-APPs, the b-Amyloid peptide (b-AP) is an internal peptide that begins about 99 residues from the carboxy-terminal end of the b-APP and extends from the extracellular domain into the middle of the hydrophobic, membrane spanning domain of the precursor protein. The precise mechanism whereby b-AP accumulates in brain remains poorly de ned.
Product Categories/Family for anti-b-Amyloid Peptide antibody
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Product Notes
The b-Amyloid Peptide (Catalog #AAA600139) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The b-Amyloid Peptide reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's b-Amyloid Peptide can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in ELISA, Immunohistochemistry and Western Blot. Dilution: Immunohistochemistry (alcohol-fixed, parrafin embedded, frozen): ~10ug/ml It is nessessary to pretreat the tissue with trypsin digestion. Western Blot: 0.1-0.2ug/ml ELISA: 0.5-1.0ug/ml. Researchers should empirically determine the suitability of the b-Amyloid Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "b-Amyloid Peptide, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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