Rat Interleukin 23 (IL23) ELISA Kit | IL23 elisa kit

Mini Samples Rat Interleukin 23 (IL23) ELISA Kit

Cat. Num. AAA2097860

• Reactivity: Rat
• Synonyms: Interleukin 23 (IL23); Mini Samples Rat Interleukin 23 (IL23) ELISA Kit; IL23A; P19; IL23P19; SGRF; Interleukin-23 subunit alpha; IL23 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 23 (IL23).; No significant cross-reactivity or interference between Mini Samples Interleukin 23 (IL23) and analogues was observed.
• Sequence Length: 196

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.6pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 23 (IL23) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 23 (IL23) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL23 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 23 (IL23). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 23 (IL23). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 23 (IL23), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 23 (IL23) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL23 elisa kit

Cytokine; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 18426832
• NCBI GeneID: 155140
• NCBI Accession #: NP_569094.1
• NCBI GenBank Nucleotide #: NM_130410.2
• UniProt Accession #: Q91Z84
• Molecular Weight: 21,986 Da
• NCBI Official Full Name: interleukin-23 subunit alpha
• NCBI Official Synonym Full Names: interleukin 23 subunit alpha
• NCBI Official Symbol: Il23a
• NCBI Protein Information: interleukin-23 subunit alpha
• UniProt Protein Name: Interleukin-23 subunit alpha
• UniProt Gene Name: Il23a
• UniProt Synonym Gene Names: IL-23 subunit alpha; IL-23-A; IL-23p19

NCBI Description

mouse homolog is a pro-inflammatory cytokine; high levels of expression may be associated with rheumatoid arthritis, psoriasis, and multiple sclerosis [RGD, Feb 2006]

Uniprot Description

Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis ().