Mouse Interleukin 17 (IL17) ELISA Kit | IL17 elisa kit

Mouse Interleukin 17 (IL17) Mini Samples ELISA Kit

Cat. Num. AAA2089490

• Gene Names: Il17a; Il17; Ctla8; IL-17; Ctla-8; IL-17A
• Reactivity: Mouse
• Synonyms: Interleukin 17 (IL17); Mouse Interleukin 17 (IL17) Mini Samples ELISA Kit; IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8; IL17 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Interleukin 17 (IL17).; No significant cross-reactivity or interference between Mini Samples Interleukin 17 (IL17) and analogues was observed.
• Sequence Length: 158

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 15.6-1,000pg/mL
• Sensitivity: < 5.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Interleukin 17 (IL17) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL17 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Interleukin 17 (IL17). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Interleukin 17 (IL17). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Interleukin 17 (IL17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Interleukin 17 (IL17) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL17 elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 6754324
• NCBI GeneID: 16171
• NCBI Accession #: NP_034682.1
• NCBI GenBank Nucleotide #: NM_010552.3
• UniProt Accession #: Q62386; Q60971
• Molecular Weight: 17,490 Da
• NCBI Official Full Name: interleukin-17A
• NCBI Official Synonym Full Names: interleukin 17A
• NCBI Official Symbol: Il17a
• NCBI Official Synonym Symbols: Il17; Ctla8; IL-17; Ctla-8; IL-17A
• NCBI Protein Information: interleukin-17A
• UniProt Protein Name: Interleukin-17A
• UniProt Gene Name: Il17a
• UniProt Synonym Gene Names: Ctla8; Il17; IL-17; IL-17A; CTLA-8

NCBI Description

This gene encodes a pro-inflammatory cytokine that is a member of the interleukin-17 family. The encoded protein plays a central role in host defense against diverse pathogens. The encoded protein is produced by activated T-cells and certain cell types of innate immune system. The active protein functions as either a homodimer with other interleukin-17 family members and signals through the interleukin-17 receptor to induce inflammatory cytokine production. Aberrant expression of this gene is associated with autoinflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. [provided by RefSeq, Sep 2015]

Uniprot Description

IL17A: Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of ICAM1/intracellular adhesion molecule 1 in fibroblasts. Belongs to the IL-17 family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1|1 A4

Cellular Component: cytoplasm; external side of plasma membrane; extracellular space

Molecular Function: cytokine activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding

Biological Process: cell surface receptor linked signal transduction; defense response to fungus; positive regulation of interleukin-23 production; positive regulation of osteoclast differentiation; positive regulation of transcription from RNA polymerase II promoter