Mouse Interleukin 18 Binding Protein (IL18BP) ELISA Kit | IL18BP elisa kit

Mouse Interleukin 18 Binding Protein (IL18BP) ELISA Kit

Cat. Num. AAA2018945

• Gene Names: IL18BP; IL18BPa
• Reactivity: Mouse
• Synonyms: Interleukin 18 Binding Protein (IL18BP); Mouse Interleukin 18 Binding Protein (IL18BP) ELISA Kit; IL18BPa; MC51L-53L-54L Homolog Gene Product; Tadekinig-alfa; IL18BP elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 18 Binding Protein (IL18BP).; No significant cross-reactivity or interference between Interleukin 18 Binding Protein (IL18BP) and analogues was observed.
• Sequence Length: 194

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
• Detection Range: 62.5-4000pg/mL
• Sensitivity: < 27.1pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 18 Binding Protein (IL18BP) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 18 Binding Protein (IL18BP) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for IL18BP elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 18 Binding Protein (IL18BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 18 Binding Protein (IL18BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 18 Binding Protein (IL18BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 18 Binding Protein (IL18BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for IL18BP elisa kit

Cytokine

NCBI and Uniprot Product Information

• NCBI GI #: 28278660
• NCBI GeneID: 10068
• UniProt Accession #: O95998; O95993; O96027; Q9NZA9; Q9UBR7; B3KUZ0; B7WPK4
• Molecular Weight: 17,478 Da
• NCBI Official Full Name: Interleukin 18 binding protein
• NCBI Official Synonym Full Names: interleukin 18 binding protein
• NCBI Official Symbol: IL18BP
• NCBI Official Synonym Symbols: IL18BPa
• NCBI Protein Information: interleukin-18-binding protein
• UniProt Protein Name: Interleukin-18-binding protein
• Protein Family: Interleukin-18-binding protein
• UniProt Gene Name: IL18BP
• UniProt Synonym Gene Names: IL-18BP
• UniProt Entry Name: I18BP_HUMAN

NCBI Description

The protein encoded by this gene functions as an inhibitor of the proinflammatory cytokine, IL18. It binds IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production, resulting in reduced T-helper type 1 immune responses. This protein is constitutively expressed and secreted in mononuclear cells. Elevated level of this protein is detected in the intestinal tissues of patients with Crohn's disease. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Uniprot Description

IL18BP: Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: extracellular space; extracellular region

Molecular Function: receptor antagonist activity; interleukin-18 binding

Biological Process: T-helper 1 type immune response; response to lipopolysaccharide