Rat IL-20 ELISA Kit | IL-20 elisa kit

Rat IL-20 (Interleukin 20) ELISA Kit

Cat. Num. AAA2516328

• Gene Names: IL20; IL-20; IL10D; ZCYTO10
• Reactivity: Rat
• Synonyms: IL-20; Rat IL-20 (Interleukin 20) ELISA Kit; IL-20 elisa kit

• Reactivity: Rat
• Specificity: This kit recognizes natural and recombinantRatIL-20. No significant cross-reactivity or interference between RatIL-20 and analogues was observed.
• Sequence Length: 176

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-20 elisa kit

Intended Uses: This ELISA kit applies to the invitro quantitative determination of RatIL-20concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to IL-20. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for IL-20and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain IL-20, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of IL-20.You can calculate the concentration of IL-20in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31083166
• NCBI GeneID: 50604
• NCBI Accession #: NP_061194.2
• NCBI GenBank Nucleotide #: NM_018724.3
• Molecular Weight: 17,144 Da
• NCBI Official Full Name: interleukin-20
• NCBI Official Synonym Full Names: interleukin 20
• NCBI Official Symbol: IL20
• NCBI Official Synonym Symbols: IL-20; IL10D; ZCYTO10
• NCBI Protein Information: interleukin-20; cytokine Zcyto10; four alpha helix cytokine
• UniProt Protein Name: Interleukin-20
• UniProt Gene Name: IL20
• UniProt Synonym Gene Names: ZCYTO10; IL-20
• UniProt Entry Name: IL20_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding

Biological Process: positive regulation of osteoclast differentiation; regulation of inflammatory response; positive regulation of epidermal cell differentiation; positive regulation of JAK-STAT cascade; immune response; inflammatory response; positive regulation of keratinocyte differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein