Monkey Interleukin 20 ELISA Kit | IL-20 elisa kit

Monkey Interleukin 20 ELISA Kit

Cat. Num. AAA745968

• Gene Names: IL20; IL-20; IL10D; ZCYTO10
• Reactivity: Monkey
• Synonyms: Interleukin 20; Monkey Interleukin 20 ELISA Kit; IL-20 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-20 elisa kit

Intended Uses: This IL-20 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey IL-20. This ELISA kit for research use only!

Principle of the Assay: IL-20 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-20 antibody and an IL-20-HRP conjugate. The assay sample and buffer are incubated together with IL-20-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-20 concentration since IL-20 from samples and IL-20-HRP conjugate compete for the anti-IL-20 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-20 from the sample, fewer sites are left to bind IL-20-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-20 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for IL-20 elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 31083166
• NCBI GeneID: 50604
• NCBI Accession #: NP_061194.2
• NCBI GenBank Nucleotide #: NM_018724.3
• Molecular Weight: 17,144 Da
• NCBI Official Full Name: interleukin-20
• NCBI Official Synonym Full Names: interleukin 20
• NCBI Official Symbol: IL20
• NCBI Official Synonym Symbols: IL-20; IL10D; ZCYTO10
• NCBI Protein Information: interleukin-20; cytokine Zcyto10; four alpha helix cytokine
• UniProt Protein Name: Interleukin-20
• UniProt Gene Name: IL20
• UniProt Synonym Gene Names: ZCYTO10; UNQ852/PRO1801; IL-20
• UniProt Entry Name: IL20_HUMAN

NCBI Description

The protein encoded by this gene is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through signal transducer and activator of transcription 3 (STAT3) in keratinocytes. A specific receptor for this cytokine is found to be expressed in skin and upregulated dramatically in psoriatic skin, suggesting a role for this protein in epidermal function and psoriasis. [provided by RefSeq, Jul 2008]

Uniprot Description

IL20: Cytokine that may be involved in epidermal function and psoriasis. Acts through STAT3. Belongs to the IL-10 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; extracellular region

Molecular Function: interleukin-20 receptor binding; cytokine activity; interleukin-22 receptor binding

Biological Process: positive regulation of osteoclast differentiation; positive regulation of epidermal cell differentiation; regulation of inflammatory response; positive regulation of JAK-STAT cascade; immune response; inflammatory response; positive regulation of keratinocyte differentiation; positive regulation of tyrosine phosphorylation of Stat3 protein