Rabbit HSP105 Polyclonal Antibody | anti-HSP105 antibody

HSP105 Polyclonal Antibody

Cat. Num. AAA3012848

• Gene Names: HSPH1; HSP105; HSP105A; HSP105B; NY-CO-25
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: HSP105; Polyclonal Antibody; HSP105 Polyclonal Antibody; HSPH1; HSP110; KIAA0201; Antigen NY CO 25; Antigen NY-CO-25; DKFZp686M05240; Heat shock 105kD alpha; Heat shock 105kD; Heat shock 105kD beta; Heat shock 105kDa protein 1; Heat shock 105kDa protein; Heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; Heat shock 110kDa protein; Heat shock protein 105 kDa; HS105; HSP105A; HSP105B; HSPH 1; Hsph1; NY CO 25; anti-HSP105 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HSP105 polyclonal antibody detects endogenous levels of HSP105 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 858

• Applicable Applications for anti-HSP105 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:1,000-1:2,000; ICC: 1:50-1:200; IHC: 1:50-1:200
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of Hsp105 on different lysates using anti-Hsp105 antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela)

Immunocytochemistry (ICC)

(ICC staining Hsp105 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-HSP105 antibody

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2. HSP 105 is a testis-specific and HSP 90-related protein. Research indicates that HSP 105 is specifically localized in the germ cells and may translocate into the nucleus after heat shock. It is suggested that HSP 105 may contribute to the stabilization of p53 proteins in the cytoplasm of the germ cells, preventing the potential induction of apoptosis by p53.

NCBI and Uniprot Product Information

• NCBI GI #: 42544159
• NCBI GeneID: 10808
• NCBI Accession #: NP_006635.2
• NCBI GenBank Nucleotide #: NP_006635.2
• UniProt Accession #: Q92598; Q61699; Q66HA8
• Molecular Weight: 105kDa
• NCBI Official Full Name: heat shock protein 105 kDa isoform 1
• NCBI Official Synonym Full Names: heat shock protein family H (Hsp110) member 1
• NCBI Official Symbol: HSPH1
• NCBI Official Synonym Symbols: HSP105; HSP105A; HSP105B; NY-CO-25
• NCBI Protein Information: heat shock protein 105 kDa
• UniProt Protein Name: Heat shock protein 105 kDa
• UniProt Gene Name: HSPH1
• UniProt Synonym Gene Names: HSP105; HSP110; KIAA0201
• UniProt Entry Name: HS105_HUMAN

NCBI Description

This gene encodes a member of the heat shock protein 70 family of proteins. The encoded protein functions as a nucleotide exchange factor for the molecular chaperone heat shock cognate 71 kDa protein (Hsc70). In addition, this protein plays a distinct but related role as a holdase that inhibits the aggregation of misfolded proteins, including the cystic fibrosis transmembrane conductance regulator (CFTR) protein. Elevated expression of this protein has been observed in numerous human cancers. [provided by RefSeq, Mar 2017]

Uniprot Description

HSP105: a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor. Two alternatively spliced isoforms have been described.

Protein type: Chaperone; Heat shock protein

Chromosomal Location of Human Ortholog: 13q12.3

Cellular Component: nucleoplasm; microtubule; cytoplasm; extracellular region; cytosol

Molecular Function: protein binding; ATP binding; alpha-tubulin binding

Biological Process: receptor-mediated endocytosis; positive regulation of NK T cell activation; chaperone cofactor-dependent protein folding; positive regulation of MHC class I biosynthetic process; response to unfolded protein