Typical Testing Data/Standard Curve (for reference only)
Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit | HIF2a elisa kit
Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit
Gene Names
Epas1; HLF; Hif2a; HIF2 alpha; HIF-2 alpha
Reactivity
Rat
Synonyms
Hypoxia Inducible Factor 2 Alpha (HIF2a); Rat Hypoxia Inducible Factor 2 Alpha (HIF2a) ELISA Kit; HIF2a elisa kit
Reactivity
Rat
Sequence Length
874
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
15ng/L - 3000ng/L
Sensitivity
7.15ng/L
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HIF2a elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Hypoxia Inducible Factor 2 Alpha (also known as HIF2A) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HIF2A antibody. HIF2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HIF2A Antibody is added and binds to HIF2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HIF2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat HIF2A antibody. HIF2A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HIF2A Antibody is added and binds to HIF2A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated HIF2A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat HIF2A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
96,718 Da
NCBI Official Full Name
hypoxia inducible factor 2 alpha
NCBI Official Synonym Full Names
endothelial PAS domain protein 1
NCBI Official Symbol
Epas1
NCBI Official Synonym Symbols
HLF; Hif2a; HIF2 alpha; HIF-2 alpha
NCBI Protein Information
endothelial PAS domain-containing protein 1
UniProt Protein Name
Endothelial PAS domain-containing protein 1
UniProt Gene Name
Epas1
UniProt Synonym Gene Names
Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha
NCBI Description
activates luciferase activity after transfection [RGD, Feb 2006]
Uniprot Description
Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters (). Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().
Research Articles on HIF2a
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Product Notes
The Rat HIF2a epas1 (Catalog #AAA1600624) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600624 ELISA Kit recognizes Rat HIF2a. It is sometimes possible for the material contained within the vial of "Hypoxia Inducible Factor 2 Alpha (HIF2a), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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