Mouse beta Tubulin Monoclonal Antibody | anti-TUBB antibody

Anti-beta Tubulin Alexa Fluor488

• Reactivity: Mouse, Rat, Pig, Human
• Applications: Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Synonyms: beta Tubulin; Monoclonal Antibody; Anti-beta Tubulin Alexa Fluor488; TUBB; tubulin beta; beta-Tubulin; anti-TUBB antibody

• Host: Mouse
• Reactivity: Mouse, Rat, Pig, Human
• Clonality: Monoclonal
• Isotype: Mouse IgM
• Clone Number: TU-12
• Specificity: The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.
• Purity/Purification: The purified antibody is conjugated with Alexa Fluor488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
• Form/Format: Stabilizing Tris buffered saline (TBS) solution with 15mM sodium azide
• Concentration: 0.1mg/ml (varies by lot)

• Applicable Applications for anti-TUBB antibody: Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS) (QC tested)
• Application Notes: FC/FACS: Recommended dilution: 2-5ug/ml. Intracellular staining.
• Conjugation: Alexa Fluor488
• Immunogen: Porcine brain microtubule protein MTP-1.
• Preparation and Storage: Store in the dark at 2-8 degree C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Flow Cytometry (FC/FACS)

(Separation of HeLa cells stained using anti-beta-Tubulin (TU-12) Alexa Fluor 488 antibody (concentration in sample 5 ug/ml, red-filled) from unstained HeLa cells in flow cytometry analysis (intracellular staining) of HeLa cell suspension.)

Related Product Information for anti-TUBB antibody

The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The beta-tubulin (relative molecular weight around 50kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.

References

*Blume Y, Yemets A, Sheremet Y, Nyporko A, Sulimenko V, Sulimenko T, Draber P: Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol. 2010 Feb 18;10(1):29. [Epub ahead of print], URL: https://www.ncbi.nlm.nih.gov/pubmed/20167106itool,*Smertenko A, Blume Y, Viklický V, Dráber P: Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies. Eur J Cell Biol. 1997 Feb;72(2):104-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/9157006itool=,*Draber P, Draberova E, Viklicky V.: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1714437dopt=A
*Linhartova I, Draber P, Draberova E, Viklický V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 (Pt 3):919-24., URL: https://www.ncbi.nlm.nih.gov/pubmed/1281983dopt=A

NCBI and Uniprot Product Information

• NCBI GI #: 13562114
• NCBI GeneID: 81027
• NCBI Accession #: NP_110400
• NCBI GenBank Nucleotide #: NM_030773
• UniProt Accession #: Q9H4B7
• Molecular Weight: 50,327 Da
• NCBI Official Full Name: tubulin beta-1 chain
• NCBI Official Synonym Full Names: tubulin, beta 1 class VI
• NCBI Official Symbol: TUBB1
• NCBI Protein Information: tubulin beta-1 chain; beta tubulin 1, class VI; class VI beta-tubulin
• UniProt Protein Name: Tubulin beta-1 chain
• Protein Family: Tubulin
• UniProt Gene Name: TUBB1
• UniProt Entry Name: TBB1_HUMAN