Rat Hyaluronic Acid (HA) ELISA Kit | HA elisa kit

Rat Hyaluronic Acid (HA) ELISA Kit

Cat. Num. AAA267539

• Gene Names: LYVE1; HAR; XLKD1; LYVE-1; CRSBP-1
• Reactivity: Rat
• Synonyms: Hyaluronic Acid (HA); Rat Hyaluronic Acid (HA) ELISA Kit; HA elisa kit

• Reactivity: Rat
• Sequence Length: 322

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for HA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Rat HA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 10800122
• NCBI GeneID: 10894
• Molecular Weight: 35,213 Da
• NCBI Official Full Name: hyaluronic acid receptor
• NCBI Official Synonym Full Names: lymphatic vessel endothelial hyaluronan receptor 1
• NCBI Official Symbol: LYVE1
• NCBI Official Synonym Symbols: HAR; XLKD1; LYVE-1; CRSBP-1
• NCBI Protein Information: lymphatic vessel endothelial hyaluronic acid receptor 1; extracellular link domain containing 1; extracellular link domain-containing 1; extracellular link domain-containing protein 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1
• UniProt Protein Name: Lymphatic vessel endothelial hyaluronic acid receptor 1
• Protein Family: Hemagglutinin
• UniProt Gene Name: LYVE1
• UniProt Synonym Gene Names: CRSBP1; HAR; XLKD1; UNQ230/PRO263; LYVE-1; CRSBP-1
• UniProt Entry Name: LYVE1_HUMAN

NCBI Description

This gene encodes a type I integral membrane glycoprotein. The encoded protein acts as a receptor and binds to both soluble and immobilized hyaluronan. This protein may function in lymphatic hyaluronan transport and have a role in tumor metastasis. [provided by RefSeq, Jul 2008]

Uniprot Description

XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: transmembrane receptor activity; hyaluronic acid binding; receptor activity

Biological Process: anatomical structure morphogenesis; glycosaminoglycan metabolic process; transport; cell-matrix adhesion; carbohydrate metabolic process; response to wounding; pathogenesis; signal transduction; cell motility; hyaluronan metabolic process; hyaluronan catabolic process