Rabbit HINT1 Polyclonal Antibody | anti-HINT1 antibody

HINT1 Antibody

Cat. Num. AAA9405630

• Gene Names: HINT1; HINT; NMAN; PKCI-1; PRKCNH1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: HINT1; Polyclonal Antibody; HINT1 Antibody; Histidine triad nucleotide-binding protein 1; HINT; PKC inhibitory protein; PKCI-1; anti-HINT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 126

• Applicable Applications for anti-HINT1 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 17 amino acid peptide near the center of the human HINT1.
• Target Name: HINT1
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of HINT1 in Jurkat lysate with HINT1 antibody at (A) 1 and (B) 2 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of HINT1 in Jurkat cells with HINT1 antibody at 2.5 ug/mL.)

Related Product Information for anti-HINT1 antibody

Histidine triad nucleotide-binding protein 1 (HINT1) is a member of the histidine triad (HIT) protein family, a group of small nucleotide-binding and -hydrolyzing proteins. HINT1 interacts with several diverse proteins and has been suggested to have tumor suppressive activities. HINT1 catalyzes the hydrolysis of adenosine 5'-monophoramidate substrates such as AMP-morpholidate, but its enzymatic function does not appear to play a role in its tumor suppression. Recent experiments demonstrate that HINT1 forms a complex with POSH and JNK in vivo, inhibiting AP-1 activity and the phosphorylation of c-Jun, and this action could contribute to the tumor suppressor activity of HINT1. Other studies raise the possibility of HINT1 as a candidate gene for schizophrenia.

Product Categories/Family for anti-HINT1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4885413
• NCBI GeneID: 3094
• NCBI Accession #: NP_005331
• NCBI GenBank Nucleotide #: NM_005340.6
• Molecular Weight: 13,802 Da
• NCBI Official Full Name: histidine triad nucleotide-binding protein 1
• NCBI Official Synonym Full Names: histidine triad nucleotide binding protein 1
• NCBI Official Symbol: HINT1
• NCBI Official Synonym Symbols: HINT; NMAN; PKCI-1; PRKCNH1
• NCBI Protein Information: histidine triad nucleotide-binding protein 1
• UniProt Protein Name: Histidine triad nucleotide-binding protein 1
• Protein Family: Adenylylsulfatase
• UniProt Gene Name: HINT1
• UniProt Synonym Gene Names: HINT; PKCI1; PRKCNH1; PKCI-1
• UniProt Entry Name: HINT1_HUMAN

NCBI Description

The protein encoded by this gene can hydrolyze substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester, and AMP-NH2. The encoded protein interacts with these substrates via a histidine triad motif, which is part of the loop that binds to the substrate. This gene has been found to be a tumor suppressing gene. Several transcript variants, but only one of them protein-coding, have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

HINT1: Hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha- acetyl lysine methyl ester and AMP-NH2. Belongs to the HINT family.

Protein type: EC 3.-.-.-; Cytoskeletal; Hydrolase

Chromosomal Location of Human Ortholog: 5q31.2

Cellular Component: cytoskeleton; cytoplasm; histone deacetylase complex; plasma membrane; nucleus

Molecular Function: protein kinase C binding; hydrolase activity; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; purine ribonucleotide catabolic process; signal transduction

Disease: Neuromyotonia And Axonal Neuropathy, Autosomal Recessive