Rat Steroid 17-alpha-hydroxylase/17,20 lyase ELISA Kit | Cyp17a1 elisa kit

Rat Steroid 17-alpha-hydroxylase/17,20 lyase ELISA Kit

Cat. Num. AAA764052

• Gene Names: CYP17A1; CPT7; CYP17; S17AH; P450C17
• Reactivity: Rat
• Synonyms: Steroid 17-alpha-hydroxylase/17; 20 lyase; Rat Steroid 17-alpha-hydroxylase/17; 20 lyase ELISA Kit; Cyp17a1/Steroid 17-alpha-hydroxylase/17; 20 lyase/Cytochrome P450-C17/Cytochrome P450c17/Cytochrome P450-C17/Cytochrome P450c17/CYPXVII/Cytochrome P450 17A1/; Cyp17a1 elisa kit

• Reactivity: Rat
• Sequence Length: 508

• Assay Type: Sandwich
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Cyp17a1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4503195
• NCBI GeneID: 1586
• NCBI Accession #: NP_000093.1
• NCBI GenBank Nucleotide #: NM_000102.3
• Molecular Weight: 57,371 Da
• NCBI Official Full Name: steroid 17-alpha-hydroxylase/17,20 lyase
• NCBI Official Synonym Full Names: cytochrome P450 family 17 subfamily A member 1
• NCBI Official Symbol: CYP17A1
• NCBI Official Synonym Symbols: CPT7; CYP17; S17AH; P450C17
• NCBI Protein Information: steroid 17-alpha-hydroxylase/17,20 lyase
• UniProt Protein Name: Steroid 17-alpha-hydroxylase/17,20 lyase
• Protein Family: Steroid 17-alpha-hydroxylase/17,20 lyase
• UniProt Gene Name: CYP17A1
• UniProt Synonym Gene Names: CYP17; S17AH; Cytochrome P450c17
• UniProt Entry Name: CP17A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. It has both 17alpha-hydroxylase and 17,20-lyase activities and is a key enzyme in the steroidogenic pathway that produces progestins, mineralocorticoids, glucocorticoids, androgens, and estrogens. Mutations in this gene are associated with isolated steroid-17 alpha-hydroxylase deficiency, 17-alpha-hydroxylase/17,20-lyase deficiency, pseudohermaphroditism, and adrenal hyperplasia. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP17A1: Conversion of pregnenolone and progesterone to their 17- alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction. Involved in sexual development during fetal life and at puberty. Defects in CYP17A1 are the cause of adrenal hyperplasia type 5 (AH5). AH5 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: salt wasting (SW, the most severe type), simple virilizing (SV, less severely affected patients), with normal aldosterone biosynthesis, non-classic form or late onset (NC or LOAH), and cryptic (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - C21-steroid hormone; Oxidoreductase; EC 4.1.2.30; EC 1.14.99.9

Chromosomal Location of Human Ortholog: 10q24.3

Cellular Component: axon; cell soma; endoplasmic reticulum; endoplasmic reticulum membrane; mitochondrion

Molecular Function: 17-alpha-hydroxyprogesterone aldolase activity; heme binding; iron ion binding; oxygen binding; steroid 17-alpha-monooxygenase activity

Biological Process: adrenal gland development; androgen biosynthetic process; biphenyl metabolic process; dibenzo-p-dioxin metabolic process; glucocorticoid biosynthetic process; hippocampus development; hormone biosynthetic process; Leydig cell differentiation; ovulation; phenol metabolic process; phthalate metabolic process; progesterone metabolic process; response to acetate; response to cAMP; response to cytokine stimulus; response to drug; response to herbicide; response to insecticide; response to ionizing radiation; response to methylmercury; response to nutrient levels; response to retinoic acid; response to steroid hormone stimulus; sex differentiation; steroid biosynthetic process; steroid metabolic process; sterol metabolic process; xenobiotic metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 17-alpha-hydroxylase Deficiency