Rat Glutamine synthetase (GS) ELISA Kit | GS elisa kit

Rat Glutamine synthetase (GS) ELISA Kit

Cat. Num. AAA2023181

• Gene Names: GLUL; GS; GLNS; PIG43; PIG59
• Reactivity: Rat
• Synonyms: Glutamine synthetase (GS); Rat Glutamine synthetase (GS) ELISA Kit; GLNS; GLUL; Glutamate-Ammonia Ligase; Glutamate decarboxylase; GS elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamine synthetase (GS).; No significant cross-reactivity or interference between Glutamine synthetase (GS) and analogues was observed.
• Sequence Length: 373

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 15.62-1000pg/mL
• Sensitivity: < 6.2pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamine synthetase (GS) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamine synthetase (GS) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GS elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamine synthetase (GS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamine synthetase (GS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamine synthetase (GS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamine synthetase (GS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GS elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 74271837
• NCBI GeneID: 2752
• NCBI Accession #: NP_001028216.1
• NCBI GenBank Nucleotide #: NM_001033044.3
• UniProt Accession #: P15104; Q499Y9; Q5T9Z1; Q7Z3W4; Q8IZ17
• Molecular Weight: 42,064 Da
• NCBI Official Full Name: glutamine synthetase
• NCBI Official Synonym Full Names: glutamate-ammonia ligase
• NCBI Official Symbol: GLUL
• NCBI Official Synonym Symbols: GS; GLNS; PIG43; PIG59
• NCBI Protein Information: glutamine synthetase
• UniProt Protein Name: Glutamine synthetase
• UniProt Gene Name: GLUL
• UniProt Synonym Gene Names: GLNS; GS
• UniProt Entry Name: GLNA_HUMAN

NCBI Description

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

GLUL: This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner. Essential for proliferation of fetal skin fibroblasts. Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD). CSGD is a rare developmental disorder with severe brain malformation resulting in multi-organ failure and neonatal death. Glutamine is largely absent from affected patients serum, urine and cerebrospinal fluid. Belongs to the glutamine synthetase family.

Protein type: EC 6.3.1.2; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - alanine, aspartate and glutamate; Ligase; Energy Metabolism - nitrogen; EC 4.1.1.15

Chromosomal Location of Human Ortholog: 1q31

Cellular Component: protein complex; mitochondrion; rough endoplasmic reticulum; cytoplasm; perikaryon; nerve terminal; cytosol; nucleus

Molecular Function: glutamate-ammonia ligase activity; identical protein binding; dynein light chain binding; glutamate binding; glutamate decarboxylase activity; manganese ion binding; magnesium ion binding; ATP binding

Biological Process: cell proliferation; glutamate catabolic process; synaptic transmission; glutamine biosynthetic process; response to glucose stimulus; neurotransmitter uptake; positive regulation of insulin secretion; amino acid biosynthetic process; cellular response to starvation; positive regulation of synaptic transmission, glutamatergic; protein homooligomerization; positive regulation of epithelial cell proliferation

Disease: Glutamine Deficiency, Congenital