Human Glutamine synthetase (GS) RTU ELISA Kit | GS rtu elisa kit

Human Glutamine synthetase (GS) ELISA Kit

Cat. Num. AAA4503775

• Gene Names: GLUL; GS; GLNS; PIG43; PIG59
• Reactivity: Human
• Synonyms: Glutamine synthetase (GS); Human Glutamine synthetase (GS) ELISA Kit; GLNS; GLUL; Glutamate-Ammonia Ligase; Glutamate decarboxylase; GS rtu elisa kit

• Reactivity: Human
• Sequence Length: 373

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cerebrospinal Fluid or Other Biological Fluids.
• Detection Range: 15.6-1000pg/mL
• Sensitivity: 5.4pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GS rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to GS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of GS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 74271837
• NCBI GeneID: 2752
• NCBI Accession #: NP_001028216.1
• NCBI GenBank Nucleotide #: NM_001033044.3
• UniProt Accession #: P15104; Q499Y9; Q5T9Z1; Q7Z3W4; Q8IZ17
• Molecular Weight: 42,064 Da
• NCBI Official Full Name: glutamine synthetase
• NCBI Official Synonym Full Names: glutamate-ammonia ligase
• NCBI Official Symbol: GLUL
• NCBI Official Synonym Symbols: GS; GLNS; PIG43; PIG59
• NCBI Protein Information: glutamine synthetase
• UniProt Protein Name: Glutamine synthetase
• UniProt Gene Name: GLUL
• UniProt Synonym Gene Names: GLNS; GS

NCBI Description

The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

Uniprot Description

This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (). Essential for proliferation of fetal skin fibroblasts.