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Typical Testing Data/Standard Curve (for reference only)

Rat Galectin 9 (GAL9) ELISA Kit | GAL9 elisa kit

Rat Galectin 9 (GAL9) ELISA Kit

Gene Names
LGALS9; HUAT; LGALS9A
Reactivity
Rat
Synonyms
Galectin 9 (GAL9); Rat Galectin 9 (GAL9) ELISA Kit; LGALS9; Ecalectin; Lectin; Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21; GAL9 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of GAL9. No significant cross-reactivity or interference between GAL9 and analogues was observed.
Sequence Length
323
Samples
Rat serum, plasma and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
7.8-500pg/mL
Sensitivity
<2.8pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level GAL9 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level GAL9 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GAL9 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GAL9 in rat serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to GAL9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GAL9. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GAL9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of GAL9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GAL9 elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,888 Da
NCBI Official Full Name
galectin-9 isoform short
NCBI Official Synonym Full Names
lectin, galactoside-binding, soluble, 9
NCBI Official Symbol
LGALS9
NCBI Official Synonym Symbols
HUAT; LGALS9A
NCBI Protein Information
galectin-9
UniProt Protein Name
Galectin-9
Protein Family
UniProt Gene Name
LGALS9
UniProt Synonym Gene Names
Gal-9
UniProt Entry Name
LEG9_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by this gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

galectin-9: Binds galactosides. Has high affinity for the Forssman pentasaccharide. May play a role in thymocyte-epithelial interactions relevant to the biology of the thymus. Inhibits cell proliferation. It is a ligand for HAVCR2/TIM3. Induces T-helper type 1 lymphocyte (Th1) death. Isoform Short acts as an eosinophil chemoattractant. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: cytoplasm

Molecular Function: signal transducer activity; galactose binding; carbohydrate binding

Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; signal transduction

Research Articles on GAL9

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Product Notes

The Rat GAL9 lgals9 (Catalog #AAA2024112) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024112 ELISA Kit recognizes Rat GAL9. It is sometimes possible for the material contained within the vial of "Galectin 9 (GAL9), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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