Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit MAPT/Tau Polyclonal Antibody | anti-MAPT/Tau antibody
Anti-MAPT/Tau Antibody (C-Terminus)
Reactivity
HumanPredicted: Mouse, Dog, Bovine, Rat, Pig
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Immunoaffinity Purified
Synonyms
MAPT/Tau; Polyclonal Antibody; Anti-MAPT/Tau Antibody (C-Terminus); Rabbit Polyclonal to Human MAPT/Tau; MAPT; FTDP-17; MSTD; Neurofibrillary tangle protein; PHF-tau; MTBT2; PPND; DDPAC; MAPTL; MTBT1; Paired helical filament-tau; TAU; anti-MAPT/Tau antibody
Host
Rabbit
Reactivity
Human
Predicted: Mouse, Dog, Bovine, Rat, Pig
Predicted: Mouse, Dog, Bovine, Rat, Pig
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of TAU protein.
Purity/Purification
Immunoaffinity Purified
Form/Format
PBS, pH 7.3, 0.01% sodium azide, 30% glycerol.
Applicable Applications for anti-MAPT/Tau antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ICC: 1:100-1:500
IHC-P: 1:100
IP: 1:10-1:100
WB: 1:500-1:1000
IHC-P: 1:100
IP: 1:10-1:100
WB: 1:500-1:1000
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-terminal region of human TAU.
Conjugation
Unconjugated
Epitope
C-Terminus
Preparation and Storage
Store at -20 degree C.
Aliquot to avoid freeze/thaw cycles.
Aliquot to avoid freeze/thaw cycles.
Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunohistochemistry (IHC)
(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))
Immunofluorescence (IF)
(Immunofluorescent analysis of TAU staining in SKNSH cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Immunofluorescence (IF)
(Immunofluorescent analysis of TAU staining in SKNSH cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Western Blot (WB)
(Western blot analysis of TAU expression in SKNSH (A); mouse brain (B); U87MG (C); SHSY5Y (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of TAU expression in SKNSH (A); mouse brain (B); U87MG (C); SHSY5Y (D) whole cell lysates.)
Related Product Information for anti-MAPT/Tau antibody
Tau promotes microtubule assembly and stability, and might be involvedin the establishment and maintenance of neuronal polarity. the C-terminus binds axonal microtubules while the N-terminus binds neuralplasma membrane components, suggesting that tau functions as a linkerprotein between both. Axonal polarity is predetermined by taulocalization (in the neuronal cell) in the domain of the cell body defined by the centrosome.
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Product Notes
The MAPT/Tau (Catalog #AAA2401581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MAPT/Tau Antibody (C-Terminus) reacts with Human Predicted: Mouse, Dog, Bovine, Rat, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's MAPT/Tau can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB). ICC: 1:100-1:500 IHC-P: 1:100 IP: 1:10-1:100 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the MAPT/Tau for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPT/Tau, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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