Rat Furin (FUR) ELISA Kit | FUR elisa kit

Rat Furin (FUR) ELISA Kit

Cat. Num. AAA2024952

• Gene Names: FURIN; FUR; PACE; SPC1; PCSK3
• Reactivity: Rat
• Synonyms: Furin (FUR); Rat Furin (FUR) ELISA Kit; PACE; PCSK3; SPC1; Paired Basic Amino Acid Cleaving Enzyme; Dibasic-processing enzyme; FUR elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Furin (FUR).; No significant cross-reactivity or interference between Furin (FUR) and analogues was observed.
• Sequence Length: 794

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Furin (FUR) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Furin (FUR) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FUR elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Furin (FUR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Furin (FUR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Furin (FUR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Furin (FUR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for FUR elisa kit

Enzyme & Kinase; Metabolic pathway; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 4505579
• NCBI GeneID: 5045
• NCBI Accession #: NP_002560.1
• NCBI GenBank Nucleotide #: NM_002569.3
• Molecular Weight: 86,678 Da
• NCBI Official Full Name: furin preproprotein
• NCBI Official Synonym Full Names: furin (paired basic amino acid cleaving enzyme)
• NCBI Official Symbol: FURIN
• NCBI Official Synonym Symbols: FUR; PACE; SPC1; PCSK3
• NCBI Protein Information: furin
• UniProt Protein Name: Furin
• Protein Family: Ferric uptake regulation protein
• UniProt Gene Name: FURIN
• UniProt Synonym Gene Names: FUR; PACE; PCSK3; PACE
• UniProt Entry Name: FURIN_HUMAN

NCBI Description

This gene encodes a member of the subtilisin-like proprotein convertase family, which includes proteases that process protein and peptide precursors trafficking through regulated or constitutive branches of the secretory pathway. It encodes a type 1 membrane bound protease that is expressed in many tissues, including neuroendocrine, liver, gut, and brain. The encoded protein undergoes an initial autocatalytic processing event in the ER and then sorts to the trans-Golgi network through endosomes where a second autocatalytic event takes place and the catalytic activity is acquired. The product of this gene is one of the seven basic amino acid-specific members which cleave their substrates at single or paired basic residues. Some of its substrates include proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor. It is also thought to be one of the proteases responsible for the activation of HIV envelope glycoproteins gp160 and gp140 and may play a role in tumor progression. This gene is located in close proximity to family member proprotein convertase subtilisin/kexin type 6 and upstream of the FES oncogene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

FURIN: Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Belongs to the peptidase S8 family. Furin subfamily.

Protein type: Membrane protein, integral; Protease; Cell surface; EC 3.4.21.75; Vesicle

Chromosomal Location of Human Ortholog: 15q26.1

Cellular Component: Golgi membrane; extracellular space; trans-Golgi network transport vesicle; cell surface; membrane; Golgi lumen; plasma membrane; integral to membrane; trans-Golgi network; lipid raft

Molecular Function: serine-type endopeptidase inhibitor activity; peptidase activity; protease binding; serine-type endopeptidase activity; metal ion binding; endopeptidase activity; nerve growth factor binding; peptide binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; viral reproduction; peptide hormone processing; regulation of signal transduction; pathogenesis; viral infectious cycle; proteolysis; positive regulation of membrane protein ectodomain proteolysis; peptide biosynthetic process; extracellular matrix disassembly; nerve growth factor processing; transforming growth factor beta receptor signaling pathway; regulation of endopeptidase activity; negative regulation of nerve growth factor production; secretion by cell; Notch signaling pathway; signal peptide processing; virus assembly; post-translational protein modification; peptidyl-glutamic acid carboxylation; nerve growth factor production; negative regulation of transforming growth factor-beta1 production; collagen catabolic process; cell proliferation; cellular protein metabolic process; viral protein processing; regulation of protein catabolic process; protein processing; negative regulation of low-density lipoprotein receptor catabolic process