Rat Follistatin (FS) ELISA Kit | FS elisa kit

Rat Follistatin (FS) ELISA Kit

Cat. Num. AAA2024780

• Gene Names: FST; FS
• Reactivity: Rat
• Synonyms: Follistatin (FS); Rat Follistatin (FS) ELISA Kit; FS elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of follistatin. No significant cross-reactivity or interference between follistatin and analogues was observed.
• Sequence Length: 317

• Samples: Rat serum, plasma, tissue homogenates and other biological fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: The minimum detectable dose of follistatin is typically less than 0.57ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level follistatin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level follistatin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of follistatin in rat serum, plasma, tissue homogenates and other biological fluids.
• Preparation and Storage: For unopened kit, all the reagents should be kept according to the label on each vial. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 degree C while others should be at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FS elisa kit

Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to follistatin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to follistatin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain follistatin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of follistatin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 182721
• NCBI GeneID: 10468
• Molecular Weight: 34,803 Da
• NCBI Official Full Name: follistatin
• NCBI Official Synonym Full Names: follistatin
• NCBI Official Symbol: FST
• NCBI Official Synonym Symbols: FS
• NCBI Protein Information: follistatin; activin-binding protein; follistatin isoform FST317
• UniProt Protein Name: Follistatin
• Protein Family: Follistatin
• UniProt Gene Name: FST
• UniProt Synonym Gene Names: FS
• UniProt Entry Name: FST_HUMAN

NCBI Description

Follistatin is a single-chain gonadal protein that specifically inhibits follicle-stimulating hormone release. The single FST gene encodes two isoforms, FST317 and FST344 containing 317 and 344 amino acids respectively, resulting from alternative splicing of the precursor mRNA. In a study in which 37 candidate genes were tested for linkage and association with polycystic ovary syndrome (PCOS) or hyperandrogenemia in 150 families, evidence was found for linkage between PCOS and follistatin. [provided by RefSeq, Jul 2008]

Uniprot Description

FST: Binds directly to activin and functions as an activin antagonist. Specific inhibitor of the biosynthesis and secretion of pituitary follicle stimulating hormone (FSH). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; extracellular region; nucleus

Molecular Function: heparan sulfate proteoglycan binding; signal transducer activity; protein binding; activin binding

Biological Process: hair follicle morphogenesis; keratinocyte proliferation; negative regulation of transcription from RNA polymerase II promoter; pattern specification process; odontogenesis of dentine-containing teeth; positive regulation of hair follicle development; BMP signaling pathway; negative regulation of activin receptor signaling pathway; negative regulation of cell differentiation; gamete generation; hemopoietic progenitor cell differentiation; skeletal development; female gonad development

Disease: Polycystic Ovary Syndrome 1