Rat Fibroblast Growth Factor 7 (FGF7) ELISA Kit | FGF7 elisa kit

Rat Fibroblast Growth Factor 7 (FGF7) ELISA Kit

Cat. Num. AAA2700248

• Gene Names: FGF7; KGF; HBGF-7
• Reactivity: Rat
• Synonyms: Fibroblast Growth Factor 7 (FGF7); Rat Fibroblast Growth Factor 7 (FGF7) ELISA Kit; KGF; HBGF7; Keratinocyte Growth Factor; Heparin-binding growth factor 7; FGF7 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of FGF7. No significant cross-reactivity or interference between FGF7 and analogues was observed.
• Sequence Length: 194

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 62.5-1,000pg/mL
• Sensitivity: < 21.6pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FGF7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FGF7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for FGF7 elisa kit

Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of FGF7 in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to FGF7 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled FGF7 and unlabeled FGF7 (Standards or samples) with the pre-coated antibody specific to FGF7. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of FGF7 in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of FGF7 in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4503705
• NCBI GeneID: 2252
• NCBI Accession #: NP_002000.1
• NCBI GenBank Nucleotide #: NM_002009.3
• UniProt Accession #: P21781; Q6FGV5; Q96FG5; H0YNY5
• Molecular Weight: 11,520 Da
• NCBI Official Full Name: fibroblast growth factor 7
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: fibroblast growth factor 7
• UniProt Protein Name: Fibroblast growth factor 7
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF; FGF-7; HBGF-7

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: extracellular region; Golgi apparatus

Molecular Function: 1-phosphatidylinositol-3-kinase activity; chemoattractant activity; fibroblast growth factor receptor binding; growth factor activity; heparin binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity

Biological Process: actin cytoskeleton reorganization; epidermis development; fibroblast growth factor receptor signaling pathway; hair follicle morphogenesis; MAPK cascade; mesenchymal cell proliferation; peptidyl-tyrosine phosphorylation; phosphatidylinositol phosphorylation; phosphoinositide-mediated signaling; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of epithelial cell proliferation; positive regulation of GTPase activity; positive regulation of keratinocyte migration; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription, DNA-templated; regulation of phosphatidylinositol 3-kinase signaling; response to wounding; signal transduction