Typical Testing Data/Standard Curve (for reference only)
Rat Fibroblast Growth Factor 21 (FGF21) ELISA Kit | FGF21 elisa kit
Rat Fibroblast Growth Factor 21 (FGF21) Wide-range ELISA Kit
Reactivity
Rat
Applications
ELISA
Synonyms
Fibroblast Growth Factor 21 (FGF21); Rat Fibroblast Growth Factor 21 (FGF21) Wide-range ELISA Kit; Fibroblast Growth Factor 21; FGF21 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Wide-range Fibroblast Growth Factor 21 (FGF21).
No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
No significant cross-reactivity or interference between Wide-range Fibroblast Growth Factor 21 (FGF21) and analogues was observed.
Sequence Length
208
Applicable Applications for FGF21 elisa kit
ELISA (EIA)
Assay Type
Competitive Inhibition
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
12.35-1,000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 4.77pg/mL
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Wide-range Fibroblast Growth Factor 21 (FGF21) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Test Principle
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Wide-range Fibroblast Growth Factor 21 (FGF21) and unlabeled Wide-range Fibroblast Growth Factor 21 (FGF21) (Standards or samples) with the pre-coated antibody specific to Wide-range Fibroblast Growth Factor 21 (FGF21). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Wide-range Fibroblast Growth Factor 21 (FGF21) in the sample.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
fibroblast growth factor 21
NCBI Official Synonym Full Names
fibroblast growth factor 21
NCBI Official Symbol
Fgf21
NCBI Protein Information
fibroblast growth factor 21
UniProt Protein Name
Fibroblast growth factor
Protein Family
UniProt Gene Name
Fgf21
UniProt Synonym Gene Names
FGF
NCBI Description
member of the fibroblast growth factor (FGF) family; implicated in activities including embryonic development, cell growth, morphogenesis, tissue growth, and tumor growth and invasion [RGD, Feb 2006]
Research Articles on FGF21
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Product Notes
The Rat FGF21 fgf21 (Catalog #AAA2709314) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2709314 ELISA Kit recognizes Rat FGF21. AAA Biotech's Fibroblast Growth Factor 21 (FGF21) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the FGF21 fgf21 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Fibroblast Growth Factor 21 (FGF21), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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