Mouse Fibroblast Growth Factor 15 (FGF15) ELISA Kit | FGF15 elisa kit

Mouse Fibroblast Growth Factor 15 (FGF15) High Sensitive ELISA Kit

Cat. Num. AAA2708124

• Gene Names: Fgf15; FGF19
• Reactivity: Mouse
• Applications: ELISA
• Synonyms: Fibroblast Growth Factor 15 (FGF15); Mouse Fibroblast Growth Factor 15 (FGF15) High Sensitive ELISA Kit; Fibroblast Growth Factor 15; FGF15 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Fibroblast Growth Factor 15 (FGF15).; No significant cross-reactivity or interference between High Sensitive Fibroblast Growth Factor 15 (FGF15) and analogues was observed.
• Sequence Length: 218

• Applicable Applications for FGF15 elisa kit: ELISA (EIA)
• Assay Type: Competitive Inhibition
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 6.25-100pg/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 2.85pg/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 15 (FGF15) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Fibroblast Growth Factor 15 (FGF15) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Fibroblast Growth Factor 15 (FGF15) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Fibroblast Growth Factor 15 (FGF15) and unlabeled High Sensitive Fibroblast Growth Factor 15 (FGF15) (Standards or samples) with the pre-coated antibody specific to High Sensitive Fibroblast Growth Factor 15 (FGF15). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 15 (FGF15) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Fibroblast Growth Factor 15 (FGF15) in the sample.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 6679777
• NCBI GeneID: 14170
• NCBI Accession #: NP_032029.1
• NCBI GenBank Nucleotide #: NP_032029.1
• UniProt Accession #: O35622
• NCBI Official Full Name: fibroblast growth factor 15
• NCBI Official Synonym Full Names: fibroblast growth factor 15
• NCBI Official Symbol: Fgf15
• NCBI Official Synonym Symbols: FGF19
• NCBI Protein Information: fibroblast growth factor 15
• UniProt Protein Name: Fibroblast growth factor 15
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: Fgf15
• UniProt Synonym Gene Names: FGF-15
• UniProt Entry Name: FGF15_MOUSE

Uniprot Description

FGF19: Involved in the suppression of bile acid biosynthesis through down-regulation of CYP7A1 expression, following positive regulation of the JNK and ERK1/2 cascades. Stimulates glucose uptake in adipocytes. Activity requires the presence of KLB and FGFR4. Belongs to the heparin-binding growth factors family.

Protein type: Cytokine; Secreted; Secreted, signal peptide; Motility/polarity/chemotaxis; Cell development/differentiation

Cellular Component: extracellular region

Molecular Function: protein binding; growth factor activity; fibroblast growth factor receptor binding; receptor binding

Biological Process: positive regulation of glucose import; fibroblast growth factor receptor signaling pathway; heart development; positive regulation of cell proliferation; positive regulation of JNK cascade; positive regulation of protein amino acid phosphorylation; neural crest cell migration